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Transition Temperature (0):
Recent article
Transition Temperature
Abstract
We describe the potential of estimating melting temperature (T(m)) for nonstandard oligonucleotides by using the correlation of the experimental T(m) with the calculated duplex binding energy (BE) for oligonucleotides of a given length. This method has been automated into a standardized molecular dy...
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PMID: 21702481
PDF is available here.
PDF is available here.
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Fungal Biology (115)8 2011
Abstract
We applied one of the most sensitive and rapid diagnostic screening method so far developed, called High-Resolution Melting Analysis (HRMA), to detect D. pinea in Austrian pine (Pinus nigra). HRMA exploits differences in the melting behaviour of PCR products to rapidly identify DNA sequence variants...
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PMID: 21802051
PDF is available here.
PDF is available here.
Abstract
We investigate the light-triggered release of DNA from two types of nanoparticle substrates: Au nanoshells and Au nanorods. Both light-triggered and thermally induced release are distinctly observable from nanoshell-based complexes, with light-triggered release occurring at an ambient solution tempe...
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PMID: 21736347
PDF is available here.
PDF is available here.
Abstract
We developed a closed-tube HRM mutation detection method specific for SLC26A4 that can be used in the clinical diagnostic setting. Twenty-eight primer pairs were designed to cover all 21 SLC26A4 exons and splice junction sequences. Using the resulting amplicons, initial HRM analysis detected all 45...
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PMID: 21704276
PDF is available here.
PDF is available here.
Abstract
We describe a rapid multiplexed dual-color fluorescence resonance energy transfer (FRET) probe assay that accurately genotypes for both SNPs simultaneously. A single-nucleotide extension assay was also developed for verification of genotypes. Agreement (100%) was observed in genotype calls between t...
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PMID: 21704279
PDF is available here.
PDF is available here.
Abstract
We found binding between TR-DHPE and DPPC caused by electrostatic interactions. On average, TR-DHPE is bound to 1.2 DPPC molecules. Binding reduced the diffusion coefficient of TR-DHPE by 34% relative to unlabeled DPPC molecules. We estimate that binding would lead to a ∼ 5 °C increase in the liq...
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PMID: 21644587
PDF is available here.
PDF is available here.
Abstract
Dihydroartemisinin (DHA) is a poorly water-soluble drug that displays low bioavailability after oral administration. Attempts have been made to improve the solubility of DHA. Yet, no information is available concerning improved bioavailability. This study aimed to improve the water solubility of DHA...
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PMID: 21656361
PDF is available here.
PDF is available here.
Abstract
The loss of crystalline structure in sucrose, glucose, and fructose has been shown to be due to the kinetic process of thermal decomposition (termed apparent melting), rather than thermodynamic melting. The purpose of this research was to investigate whether or not it is possible to scan quickly enou...
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PMID: 21417276
PDF is available here.
PDF is available here.
Abstract
Pore formation and pore closure in poly(D,L-lactide-co-glycolide)-based drug delivery systems are two important processes as they control the release of the encapsulated drug. The phenomenon pore closure was investigated by studying the effects of the pH and the temperature of the re...
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PMID: 21094192
PDF is available here.
PDF is available here.
Abstract
Molecular mobility in amorphous solid biomaterials is modulated by the composition and environment (primarily temperature). Phosphorescence of the triplet probe erythrosin B was used to generate a mobility map within amorphous sucrose films doped with starch ranging from 0.001 to 0.1 g starch/g sucro...
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PMID: 21381746
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (27)2 2011
Abstract
Compared with (1.74±0.28)% of the relative mRNA expression level of TCRζ gene in TCR gene-untransduced T cells, the expression level of TCRζ gene was (1.78±0.22)% in unactivated TCR gene-transduced T cells and showed no obvious increase. While the expression of TCRζ gene arrived at (11.54±1.98...
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PMID: 21315043
PDF is available here.
PDF is available here.
Abstract
We report the synthesis and characterization of a novel cross-linked N-isopropylacrylamide-acrylic acid-hydroxyethyl methacrylate [P (NIPASM-AA-HEM)] hydrogel nanoparticles (NPs) containing amoxicillin. The aim of present study was to investigate whether these hydrogel NPs have the potential to be u...
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PMID: 21280053
PDF is available here.
PDF is available here.
Abstract
We have recently overcome for the myocilin OLF domain (myoc-OLF). Here, we report the first detailed solution biophysical characterization of myoc-OLF to gain insight into its structure and function. Myoc-OLF is stable in the presence of glycosaminoglycans, as well as in a wide pH range in buffers w...
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PMID: 21283635
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
Biological scaffolds used in tissue engineering are incorporated in vivo by a process of cellular in-growth, followed by host-mediated degradation and replacement of these scaffolds, in which phagocytic cells from the monocyte/macrophage cell lineage play a key role. The chemical deg...
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PMID: 20709200
PDF is available here.
PDF is available here.
Abstract
We previously showed by NMR and molecular modeling that the unexpectedly high binding affinity of 2'F-ANA is largely due to a C-H···F-C pseudohydrogen bond at pyrimidine-purine steps. Comparisons of the melting of duplexes with identical sequence composition but a rearranged sequence confirm that...
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PMID: 21171597
PDF is available here.
PDF is available here.
Abstract
To examine the thermal expansion of resin nanocomposites after light-curing using different light-curing units.
Four different resin nanocomposites and four different light-curing units [quartz-tungsten-halogen (QTH), light emitting diode (LED), laser, and plasma arc]...
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PMID: 21344832
PDF is available here.
PDF is available here.
Abstract
The triplet state of positronium, o-Ps, is used as a probe to characterize a starch-20% w/w sucrose matrix as a function of temperature (T). A two-step decomposition (of sucrose, and then starch) starts at 440 K as shown by a decrease in the o-Ps intensity (I(3)) and lifetime (τ(3)), the latter als...
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PMID: 20882224
PDF is available here.
PDF is available here.
Abstract
Dynamic light scattering performed on aqueous solutions of three sugars (glucose, maltose and sucrose) reveal a common pattern of sugar cluster formation with a narrow cluster size distribution. In each case, equilibrium clusters form whose size increases with increasing sugar content in an identica...
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PMID: 21230497
PDF is available here.
PDF is available here.
Abstract
Three new forms of the osteoporosis drug sodium risedronate, sodium [1-hydroxy-2-(3-pyridinyl)ethylidene]bisphosphonate, were identified and designated as the J, K, and M phases. Form J is an acetic acid disolvate with the chemical composition Na(+) [C(7) H(10) NO(7) P(2)](-) · 2CH(3) COOH, as deter...
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PMID: 20967794
PDF is available here.
PDF is available here.
Abstract
We show that despite the highly ordered structure and tacticity-dependent nature of hydrogen-bonded supramolecular polymers, the bioactivity of these polymers can be tuned through composite preparation with bioceramics. These novel supramolecular composites combine the superior processability of sup...
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PMID: 20574978
PDF is available here.
PDF is available here.
Abstract
Significant improvement of solubilization kinetics of poorly soluble vinpocetine was obtained through a mechanochemical activation process in presence of micronized crospovidone. Drug-to-polymer weight ratio and milling time variables resulted to have statistically significant impacts on the activati...
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PMID: 20839363
PDF is available here.
PDF is available here.
Abstract
Tear film osmolarity measured with the OcuSense TearLab system correlates well with the Clifton Osmometer. The new instrument has the potential to provide clinicians with a readily available clinically applicable measure, which could become the gold standard in DED....
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PMID: 20577088
PDF is available here.
PDF is available here.
Abstract
With a view to the application in oral controlled drug delivery systems (DDS), the design of new interpolyelectrolyte complexes (IPECs) between countercharged types of Eudragit EPO (EPO) and Eudragit L 100-55 (L100-55) was investigated. The formation and composition of four new IPECs between EPO and...
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PMID: 20803617
PDF is available here.
PDF is available here.
Journal of Chromatography A (1217)34 2010
Abstract
We evaluated the interface by controlling the loop temperature in a water bath at normal temperature, and investigated the sample losses by using standard samples with different boiling points. It was found that the sample loss due to evaporation in the interface was negligible for non-volatile samp...
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PMID: 20630530
PDF is available here.
PDF is available here.
Abstract
The N-(pyrrolidin-2-ethyl) glycine-based PNA (pet-PNA) backbone, with 4-amino or 4-guanidino-functionalized pyrrolidine ring, confers constrained conformational flexibility on aegPNA. The oligomers bind to the target DNA and RNA sequences with increased sequence specificity and antiparallel versus p...
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PMID: 20539879
PDF is available here.
PDF is available here.
Abstract
In this study, a new dynamic water vapor sorption gravimetry (DWVSG)-Raman spectroscopy coupled system is presented and described for the investigation of water (de)sorption-induced solid-phase transition of active pharmaceutical ingredients (APIs). The innovative characteristic of the system is the...
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PMID: 20740677
PDF is available here.
PDF is available here.
Biophysical Journal (99)4 2010
Abstract
We performed computer simulations on model proteins and found that: 1), For weakly adsorbing surfaces, unfolded conformations lose more entropy upon adsorption than folded ones. 2), The melting temperature, both in the bulk and at surfaces, decreases with increasing protein concentration because of...
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PMID: 20712999
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (59)Pt 8 2010
Abstract
DNA sequence variation analysis has divided varicella-zoster virus (VZV; Human herpesvirus 3) into distinct geographical clades: European, Asian, African and Japanese. These genotypes are becoming increasingly prevalent within regions atypical to their original source and there has been the suggesti...
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PMID: 20466839
PDF is available here.
PDF is available here.
Abstract
We previously confirmed that they conferred GCV resistance to recombinant baculoviruses (Nakano et al., J. Virol. Methods 161:223-230, 2009). Six characterized mutations, including a previously reported one that encodes a GCV-sensitive kinase-activity mutant (Isegawa et al., J. Clin. Virol. 44:15-19...
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PMID: 20083192
PDF is available here.
PDF is available here.
Pharmaceutical Research (27)8 2010
Abstract
To study crystal growth rates of amorphous griseofulvin (GSF) below its glass transition temperature (T (g)) and the effect of surface crystallization on the overall crystallization kinetics of amorphous GSF.
Amorphous GSF was generated by melt quenching. Surface and...
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PMID: 20414704
PDF is available here.
PDF is available here.
Abstract
We were able to differentiate all Borrelia species and their subtypes within particular species. RT-PCR based on Tm determination identified unique strains within the species Borreliaafzelii (Tm 66.11 degrees C), B. burgdorferi sensu stricto (Tm 68.18 degrees C), Borreliaspielmanii (Tm 59.45 degrees...
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PMID: 20451566
PDF is available here.
PDF is available here.
Bioresource Technology (101)15 2010
Abstract
Glycerol acetals from butanal, pentanal, hexanal, octanal and decanal were prepared with the use of Amberlyst-15 acid resin as catalyst. The glycerol conversion decreases with the size of the hydrocarbon chain. This fact has been associated with formation of micelles and aggregates of the aldehyde t...
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PMID: 20304633
PDF is available here.
PDF is available here.
Bioresource Technology (101)15 2010
Abstract
Physico-chemical properties of gelatin extracted from rainbow trout (Onchorhynchus mykiss) skin were optimized using response surface methodology (RSM). Central rotatable composite design was applied to study the combined effects of NaOH concentration (0.01-0.21 N), acetic acid concentration (0.01-0...
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PMID: 20346650
PDF is available here.
PDF is available here.
Chemical Communications (46)28 2010
Abstract
DNA hairpin structures can be stabilized by introduction of constraint on the sugar phosphate backbone within the unpaired loop moiety by means of dinucleotides locked with an alpha torsion angle in gauche(+) configuration.
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PMID: 20539885
PDF is available here.
PDF is available here.
Abstract
We report on the characterization of two Cold Hibernated Elastic Memory (CHEM) foams before and after two novel low-temperature sterilization techniques (plasma and ozone). Foams have different transition temperatures (T(trans)), as determined by Tandelta peaks in DMA tests, that depend on their che...
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PMID: 20407808
PDF is available here.
PDF is available here.
Abstract
These results extend the list of promising isothioureas with substantial activity in vitro and suggest that an in-depth study of toxicity, antimicrobial properties in vivo and nitric oxide synthase isoform selectivity of selected novel compounds is warranted....
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PMID: 20607486
PDF is available here.
PDF is available here.
Abstract
Two series of novel 2-butyl succinate-based Hydroxamate derivatives containing isoxazole rings were synthesized, characterized and evaluated for antibacterial activity. The synthesized compounds were found to exhibit weak to moderate inhibitory activity against Staphytlococcus aureu and Klebsiellar...
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PMID: 20607487
PDF is available here.
PDF is available here.
Abstract
Several triazenoindazoles 3a-e and triazinopyrazolopyridines 6a-i were prepared through the reaction of the corresponding 3-amino-4-chloroindazole and 3-aminopyrazolopyridine diazonium salts 2 and 5 with a number of secondary amines. All compounds were evaluated for their in vitro cytotoxic activity...
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PMID: 20607485
PDF is available here.
PDF is available here.
Abstract
The biophysical characterization of CPX-351, a liposomal formulation of cytarabine and daunorubicin encapsulated in a synergistic 5:1 molar ratio (respectively), is presented. CPX-351 is a promising drug candidate currently in two concurrent Phase 2 trials for treatment of acute myeloid leukemia. It...
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PMID: 20156541
PDF is available here.
PDF is available here.
Abstract
Series of novel peptide-bridged bis-phenanthridine derivatives as well as corresponding monomers were prepared by solid phase peptide synthesis, which allowed easy and fast tuning of compound properties. Interactions of new derivatives with double stranded DNA were strongly structure-dependent, amon...
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PMID: 20202724
PDF is available here.
PDF is available here.
Abstract
A sonochemical method has been developed to synthesize shear thickening fluid. This shear thickening fluid (STF) is composed of hard silicon dioxide nanoparticles and polyethylene glycol (PEG) liquid polymer. The combination of flow-able and hard components at a particular composition, results a mat...
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PMID: 20207575
PDF is available here.
PDF is available here.
Abstract
An aqueous dispersion of lysozyme and sodium dodecyl sulfate phase separates below the cloud point. Two liquid phases are formed. At pH < pI electrolytes change the cloud point temperatures (T(c)). These follow an inverse Hofmeister series. Anions partition asymmetrically between the two phases. At...
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PMID: 20394424
PDF is available here.
PDF is available here.
Abstract
We employed DNA-functionalized gold nanoparticles (AuNPs) as a model system and found that if the alcohol content is less than approximately 30%, more alcohol leads to a faster DNA hybridization, although with a decreased melting temperature. The generality of this observation was independently veri...
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PMID: 20402504
PDF is available here.
PDF is available here.
Biochemistry (New York) (75)5 2010
Abstract
We observed a crowding-induced compaction effect on the structure of both native state and thermally denatured state of CK in the presence of Ficoll 70, which is more obvious on the structure of the denatured ensemble compared to that of the native ensemble. Our observations qualitatively accord wit...
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PMID: 20632946
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)5 2010
Interaction of cationic surfactants with DNA detected by spectroscopic and acoustic wave techniques.
Abstract
Interaction of the cationic surfactants benzalkonium chloride and 1-hexadecylpyridinium chloride, in the concentration range 0.1 microM to 1 mM with calf thymus DNA and with short 19-mer double-stranded DNA has been examined in solution using UV absorption and fluorescent spectroscopies and at the l...
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PMID: 20419246
PDF is available here.
PDF is available here.
Abstract
A series of new mesogenic azomethine diols were successfully synthesized by condensation reactions between various chloroalkanols and N,N'-bis(4-hydroxy)-benzylidene-o-toluidine (1). The structures of these compounds were confirmed by CHN, FT-IR, (1)H-NMR, and (13)C-NMR spectrophotometer. Their ther...
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PMID: 20657475
PDF is available here.
PDF is available here.
Abstract
Biophysical Journal (98)8 2010
LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment.
Abstract
We have developed a robust and accurate assay, LCP-Tm, for measuring the thermal stability of membrane proteins embedded in an LCP matrix. In its two implementations, protein denaturation is followed either by a change in the intrinsic protein fluorescence on ligand release, or by an increase in the...
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PMID: 20409473
PDF is available here.
PDF is available here.
Abstract
Fluoroquinolone (FQ)-resistance in Clostridium difficile has been associated with mutations in the quinolone-resistance determining region (QRDR) of gyr genes. In particular, the majority of resistant clinical isolates show mutations in codon 82 of gyrA or in codon 426 of gyrB. A real-time PCR metho...
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PMID: 19837154
PDF is available here.
PDF is available here.
Abstract
Didodecyldimethylammonium bromide (DDAB) (1.0 mM) vesicles in water were investigated by differential scanning calorimetry (DSC) to highlight the existing kinetic asymmetry in the gel-liquid crystalline (LC) state transitions. The experiments were performed in the range of temperature, scan rate and...
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PMID: 20096844
PDF is available here.
PDF is available here.
Thermal analysis applied to verapamil hydrochloride characterization in pharmaceutical formulations.
Abstract
Thermogravimetry (TG) and differential scanning calorimetry (DSC) are useful techniques that have been successfully applied in the pharmaceutical industry to reveal important information regarding the physicochemical properties of drug and excipient molecules such as polymorphism, stability, purity,...
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PMID: 20428054
PDF is available here.
PDF is available here.
Microbiology (156)Pt 4 2010
Abstract
Mycoplasma gallisepticum (MG) is an economically important pathogen of poultry worldwide, causing chronic respiratory disease in chickens and turkeys. Differentiation of MG strains is critical, especially in countries where poultry flocks are vaccinated with live vaccines. In this study, oligonucleo...
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PMID: 20035007
PDF is available here.
PDF is available here.
Abstract
We have overcome these limitations by purifying wt and select glaucoma-causing mutant (D380A, I477N, I477S, K423E) forms of the OLF domain (228-504) fused to a maltose binding protein (MBP) from E. coli . Monomeric fusion proteins can be isolated in solution. To determine the relative stability of w...
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PMID: 20334347
PDF is available here.
PDF is available here.
Abstract
An alternative procedure for achieving formulation design in a high-shear wet granulation process has been developed. Particularly, a new formulation map has been proposed which describes the onset of a significant granule growth as a function of the formulation variables (diluent, dry and liquid bi...
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PMID: 19969055
PDF is available here.
PDF is available here.
Abstract
A new test for pathogenic Leptospira isolates, based on RAPD-PCR and high-resolution melt (HRM) analysis (which measures the melting temperature of amplicons in real time, using a fluorescent DNA-binding dye), has recently been developed. A characteristic profile of the amplicons can be used to defi...
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PMID: 20406582
PDF is available here.
PDF is available here.
Abstract
We develop an evaporator by using induction heating (IH) with multilayer induction coil, which is made from bare molybdenum or tungsten wire without water cooling and surrounding the pure vaporized material. We optimize the shapes of induction coil and vaporized materials and operation of rf power s...
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PMID: 20192343
PDF is available here.
PDF is available here.
Abstract
The effects of tri- and monoglycerides on phospholipid (POPC) membranes were studied using spectroscopical methods. Triolein was found to form two types of POPC-rich membranes, both with POPC or as a three-component system with monopalmitin. These two membrane types were determined as co-existing ph...
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PMID: 19962372
PDF is available here.
PDF is available here.
Abstract
Abstract
Our work demonstrates that HRM-based methods, owing to their simplicity, reliability, and speed, are useful tools for diagnosis of CDA deficiency and could be of interest for personalized medicine....
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PMID: 20010457
PDF is available here.
PDF is available here.
Pharmaceutical Biology (48)2 2010
Abstract
Four quaternary isoquinoline alkaloids, dehydrocorydalmine, jatrorrhizine, columbamine, and oxyberberine, have been isolated from the whole plant of Argemone mexicana Linn. (Papaveraceae) and their structures established by spectral evidence. This is the first report of these alkaloids (dehydrocoryd...
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PMID: 20645832
PDF is available here.
PDF is available here.