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Electrochemotherapy (0):
Recent article
Electrochemotherapy
Xenobiotica (41)5 2011
Abstract
Studies described in the recent literature support the idea that gene therapy can lead to genuine clinical benefits when mediated by plasmid delivery in conjunction with electroporation. Plasmid-mediated muscle-targeted gene transfer offers the potential of a cost-effective pharmaceutical-grade ther...
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PMID: 18370221
PDF is available here.
PDF is available here.
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Xenobiotica (41)5 2011
Abstract
We have optimized the electroporation conditions in a large mammal model, i.e. pig. The parameters optimized include electric field intensity, number of pulses, lag time between plasmid injection and electroporation, and plasmid delivery volume. Constant current pulses, between 0.4 and 0.6 A, applie...
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PMID: 18370201
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Electroporation has been used in biological laboratories for many years to transiently porate cell membranes and permit plasmid or protein transfection. It has been shown that the application of pulsed electric fields (PEFs) of defined strength will kill off larger cells and select for viable small...
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PMID: 18370208
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Xenobiotica (41)5 2011
Abstract
We discuss that the DNA delivery into skin can be greatly enhanced by topical electroporation of the DNA injection site in rabbits using a tweezer electrode. Furthermore, the immune responses following a DNA vaccine delivery by using electroporation have been explored. Electroporation shows great po...
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PMID: 18370202
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Using gene therapy to produce systemic levels of human factor IX for the treatment of hemophilia B has been clinically evaluated using viral-based vectors. The efficacy of this approach has been limited because of immune responses against the viral components. An alternative approach is to use physi...
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PMID: 18370215
PDF is available here.
PDF is available here.
Abstract
We present the first in vivo study to achieve tumor regression using a translatable, clinically relevant single needle electrode for treatment administration. Numerical models of the electric field distribution for the protocol used suggest that a 1000 V/cm field threshold is sufficient to treat a t...
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PMID: 20191380
PDF is available here.
PDF is available here.
Current Gene Therapy (10)4 2010
Abstract
In vivo electroporation (EP) is a versatile delivery method for gene transfer which can be applied to any accessible tissue. Delivery of plasmid DNA encoding therapeutic genes or cDNAs with in vivo EP has been tested extensively in preclinical melanoma models. Direct delivery to the...
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PMID: 20557286
PDF is available here.
PDF is available here.
Dermatologic Surgery (36)7 2010
Abstract
Abstract
We compared efficacy of combined treatment of males with anogenital warts given intramuscular cycloferon or cycloferon electrophoresis. Patients with manifest HPV infection (n=107) were divided into two groups. Group 1 (n=72) received intramuscular cycloferon (2 ml 12.5% solution each other day for...
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PMID: 21427998
PDF is available here.
PDF is available here.
Abstract
Treatment of recurrent or in-transit unresectable melanoma continues to be a major therapeutic challenge. Electrochemotherapy (ECT) is a therapeutic option for those patients whose lesions are not suitable for surgical resection and who have exhausted all other treatment modalities. ECT combines ele...
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PMID: 19895245
PDF is available here.
PDF is available here.
Abstract
A microfabricated cell-based testing device for electrochemotherapy (ECT) has been developed; it mimics a clinical electroporator with a circular needle array and maintains a similar electric field strength distribution. Until now, the performance between electroporators having two- and six-needle c...
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PMID: 19344145
PDF is available here.
PDF is available here.
Abstract
Abstract
The clinical stages of the tumours were stage I in 4 (45%) and stage III in 5 (55%) dogs treated by ECT; 12 (75%) dogs treated by surgery were stage I and 4 (25%) dogs were in clinical stage III. The median size of the tumours was 5.2 cm3 and 2.9 cm3 of tumours treated by surgery and ECT, respective...
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PMID: 19368125
PDF is available here.
PDF is available here.
Abstract
We conclude that electrochemotherapy should be considered as a treatment option when dealing with bleeding melanoma recurrences as well as a limb-preserving treatment....
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PMID: 19688986
PDF is available here.
PDF is available here.
Abstract
Our results demonstrate that electroporation improves an access of chlorin e6 and aluminum phthalocyanine tetrasulfonate to MH22A cells. Electroporation in combination with photosensitization significantly reduces viability of the treated cells even at low doses of photosensitizers....
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PMID: 19535883
PDF is available here.
PDF is available here.
Archivio italiano di urologia, andrologia : organo ufficiale [di] Societa italiana di ecografia urologica e nefrologica / Associazione ricerche in urologia (80)4 2008
Abstract
This article reviews intravesical application of electromotive drug administration (EMDA) for the treatment of bladder cancer and the evidence in support of intravesical passive diffusion chemotherapy in the management of non-muscle invasive bladder cancer. Two recently published randomised trials a...
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PMID: 19235432
PDF is available here.
PDF is available here.
Abstract
We hypothesized that age-dependent impairment of cutaneous wound healing in db/db diabetic mice: (a) would correlate with reduced expression of the transcription factor hypoxia-inducible factor 1alpha (HIF-1alpha) as well as its downstream target genes; and (b) could be overcome by HIF-1alpha replac...
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PMID: 18506785
PDF is available here.
PDF is available here.
BJU International (102)10 2008
Abstract
These results indicate the possibility that EP with bleomycin could be effective as an ablation therapy for prostate cancer, especially androgen-independent cancer....
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PMID: 18549430
PDF is available here.
PDF is available here.
Veterinary Journal (178)2 2008
Abstract
An 8-year-old male castrated cat was referred for sudden onset of lameness. Physical examination revealed a 1x2x1cm mass originating from a footpad of the right hind leg. A diagnosis of ganglioneuroblastoma was suggested by the tumour appearance following histopathological staining with haematoxylin...
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PMID: 17910926
PDF is available here.
PDF is available here.
Abstract
We demonstrated that the placement of electrodes giving larger electrode-tissue contact surface leads to improved electrochemotherapy outcome. Our results provide guidance on electrochemotherapy for treatment of protruding cutaneous tumors using parallel plate electrodes....
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PMID: 18783290
PDF is available here.
PDF is available here.
Abstract
We report for the first time the in vivo results of attenuation of neointimal formation using IRE. Our study shows that IRE might be able to attenuate neointimal formation after angioplasty damage in a rodent model of restenosis. This approach may open new venues in the treatment of coronary artery...
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PMID: 18713696
PDF is available here.
PDF is available here.
Archives of Dermatology (144)9 2008
Abstract
We report a case of metastatic basal cell carcinoma in which electrochemotherapy was effective in inducing local regression of skin metastases. OBSERVATIONS: A 75-year-old man presented with a pigmented, deeply infiltrating nodule in the right axilla manifesting as basal cell carcinoma with squamous...
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PMID: 18794464
PDF is available here.
PDF is available here.
Anti-Cancer Drugs (19)8 2008
Abstract
We report the first study directly comparing the efficiency and toxicity of viral [adeno-associated virus (AAV2, 5, 6) and lentivirus], nonviral (Effectene, SuperFect and Lipofectamine 2000) and physical [particle-mediated gene transfer (PMGT)] methods of gene delivery in normal mouse lung cells and...
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PMID: 18690089
PDF is available here.
PDF is available here.
Abstract
Our data show that ECT and CpG ODN synergize and induce a significant increase of the local effect and a systemic T-dependent antitumor response. Such combination constitutes a potential innovative vaccination strategy using in situ tumor-associated antigens that could eventually be translated into...
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PMID: 18259749
PDF is available here.
PDF is available here.
Abstract
The immunologic and virologic outcome of therapeutic DNA-vaccines administered during antiretroviral therapy (ART) using electroporation with or without (interleukin) IL-2 treatment was evaluated in the SIVmac239/macaque model. Rhesus macaques inoculated with pathogenic SIVmac239 were treated with A...
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PMID: 18620495
PDF is available here.
PDF is available here.
Abstract
A response was obtained in 13/14 patients (93%) after the first ECT, with a complete regression (CR) in 7 (50%). Seven patients underwent a second and three a third ECT on newly appearing and residual lesions, all achieving a response. Overall, a response was obtained in 93% metastases, with lower r...
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PMID: 18498012
PDF is available here.
PDF is available here.
Cardiovascular Research (78)3 2008
Abstract
To study the role of COMP-Ang-1 in preventing hypertension and target organ damage, a COMP-Ang-1 plasmid was electroporated into adductor muscles of 6 weeks old, pre-hypertensive, spontaneously hypertensive rats (SHRs), and the secretion of its expressed protein into the bloodstream was confirmed by...
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PMID: 18285514
PDF is available here.
PDF is available here.
Abstract
Electrochemotherapy is a quick, safe, inexpensive treatment modality that has been shown to give consistently reproducible results in the treatment of solid cutaneous and subcutaneous malignant tumours. To date, its clinical license has limited its application to a palliative setting. Future work in...
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PMID: 18393004
PDF is available here.
PDF is available here.
Ultrasonics (48)2 2008
Abstract
The present study was initiated to investigate the potential biological mechanism of cell killing effect on isolate sarcoma 180 (S180) cells induced by ultrasound activating protoporphyrin IX (PPIX). S180 cells were exposed to ultrasound for 30s duration, at a frequency of 2.2 MHz and an acoustic po...
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PMID: 18068747
PDF is available here.
PDF is available here.
Veterinary Record (162)6 2008
Abstract
Abstract
We determined an increase in hypoxic tumour area for up to 30%, which was also reflected in reduced tumour oxygenation (for 70%). According to the described mathematical model, endothelial cells lining in tumour blood vessels are exposed to a approximately 40% higher electric field than the surround...
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PMID: 18182988
PDF is available here.
PDF is available here.
Abstract
The results suggest that EMDA can improve both quality of life and sexuality in patients with therapy-refractory chronic overactive bladder....
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PMID: 19111081
PDF is available here.
PDF is available here.
Abstract
An updated review of management of nevoid basal cell carcinoma syndrome (NBCCS) is presented. An ideal treatment of NBCCS does not exist, and surgical intervention has been the most commonly used treatment, as it provides excellent cure rates. However, patients with NBCCS typically present with a la...
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PMID: 18306849
PDF is available here.
PDF is available here.
Abstract
Electropermeabilization is a method that uses electric field pulses to induce an electrically mediated reorganization of the plasma membrane of cells. Electrochemotherapy combines local or systemic administration of chemotherapeutic drugs such as bleomycin or cisplatin that have poor membrane permea...
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PMID: 18537879
PDF is available here.
PDF is available here.
Abstract
Local management of soft tissue sarcoma in humans generally involves multi-modality approaches whose cornerstones are surgery combined with radiation therapy. The usual radiation protocols are based on preoperative, intraoperative, or postoperative external beam treatment or adjuvant brachytherapy....
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PMID: 17977058
PDF is available here.
PDF is available here.
Abstract
We describe the successful treatment of a cervical metastatic spread of this neoplasia by using mitoxantrone selectively driven within the tumor cells by trains of biphasic pulses. The dog experienced tumor reduction from the first cycle of electrochemotherapy (ECT) and complete remission by the tim...
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PMID: 18396782
PDF is available here.
PDF is available here.
Abstract
We describe the successful treatment of an incompletely excised ASGC by using cisplatin selectively driven within the tumor cells by trains of biphasic pulses. The dog received two courses of electrochemotherapy 14 days apart. Neither systemic nor local toxicities were detected during the whole cour...
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PMID: 18396781
PDF is available here.
PDF is available here.
Abstract
We describe the successful treatment of a recurring fibrosarcoma in a cat by using cisplatin selectively driven within the tumor cells by trains of biphasic pulses. The cat's tumor did not recur over the following five months, however the cat did experience severe erythema at the site of previous ir...
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PMID: 19181001
PDF is available here.
PDF is available here.
Abstract
Electrochemotherapy is a combined use of certain chemotherapeutic drugs and electric pulses applied to the treated tumour nodule. Local application of electric pulses to the tumour increases drug delivery into cells, specifically at the site of electric pulse application. Drug uptake by delivery of...
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PMID: 19229171
PDF is available here.
PDF is available here.
Abstract
Abstract
We studied the effects of electroporation-mediated DNA vaccination with different electro-pulse parameters in rhesus macaques. Plasmid DNA encoding the HBV preS2-S and an adjuvant plasmid encoding a fused gene of IL-2 and IFN-gamma were injected intramuscularly followed by electroporation once a mon...
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PMID: 18370224
PDF is available here.
PDF is available here.
Abstract
This chapter provides an overview of the application of electroporation to areas other than gene delivery. These areas include the delivery of drugs and vaccines to tissues and tumors as well as into and through the skin. Achievements and limitations of electroporation in these areas are presented....
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PMID: 18370192
PDF is available here.
PDF is available here.
Abstract
Bleomycin and Interleukin 12 have been used clinically to treat tumors; however, the co-administration of Bleomycin and Interleukin 12 followed by electroporation has not been tested clinically. In this study, dogs with spontaneous head and neck tumors were treated with one co-administration of Bleo...
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PMID: 18370210
PDF is available here.
PDF is available here.
Abstract
We explore the coadministration of cyclophosphamide and IL-12 plasmid DNA followed by electroporation for treating SCCVII in mice. Cyclophosphamide, IL-12 plasmid DNA, or a combination of both was injected intramuscularly in mice bearing SCCVII tumors. The tumor growth, survival, cytokine expression...
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PMID: 18370212
PDF is available here.
PDF is available here.
Abstract
We present experimental evidence for electroporation in cardiac tissue, which occurs above a threshold of 25 V/cm as evident from propidium iodide uptake, transient diastolic depolarization, and reductions of action potential amplitude and its derivative. These electrophysiological changes can induc...
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PMID: 18370220
PDF is available here.
PDF is available here.
Abstract
We have explored and optimized intradermal electroporation as an effective way of delivering a PSA DNA vaccine. The results demonstrated that intradermal DNA vaccination using low amounts of DNA, followed by two sets of electrical pulses of different length and voltage, effectively induced PSA-speci...
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PMID: 18370222
PDF is available here.
PDF is available here.
Abstract
Twenty-five years after the publication of the first report on gene transfer in vitro in cultured cells by the means of electric pulse delivery, reversible cell electroporation for gene transfer and gene therapy (DNA electrotransfer) is at a crossroad in its development. Present knowledge on the eff...
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PMID: 18370187
PDF is available here.
PDF is available here.
Mechanism by which electroporation mediates DNA migration and entry into cells and targeted tissues.
Abstract
Cell membranes can be transiently permeabilized under application of electric pulses that allow hydrophilic therapeutic molecules, such as anticancer drugs and DNA, to enter into cells and tissues. This process, called electropermeabilization or electroporation, has been rapidly developed over the l...
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PMID: 18370188
PDF is available here.
PDF is available here.
Abstract
As the medical field moves from treatment of diseases with drugs to treatment with genes, safe and efficient gene delivery systems are needed to make this transition. One such safe, nonviral, and efficient gene delivery system is electroporation (electrogenetherapy). Exciting discoveries by using el...
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PMID: 18370193
PDF is available here.
PDF is available here.
Abstract
We will describe the main characteristics of DNA electrotransfer, including toxicity and safety issues related to this technique. We will focus on the important possible therapeutic applications of electrotransfer for systemic diseases demonstrated in animal models in the recent years, in the fields...
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PMID: 18370200
PDF is available here.
PDF is available here.
Abstract
Electroporation is a safe, efficient, and inexpensive method to transfer naked plasmid DNA into various tissues. For electroporation-mediated gene transfer to the mouse lung, a plasmid solution is delivered to the lungs via the trachea. Immediately after plasmid delivery, eight square wave pulses ar...
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PMID: 18370203
PDF is available here.
PDF is available here.
Abstract
These results suggest that EP is a useful technique for in vivo gene transfer into rat bladder smooth muscles, and that the nNOS gene transferred by this procedure functionally expresses and contributes to NO production....
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PMID: 18370204
PDF is available here.
PDF is available here.
Abstract
Interleukin 12 (IL-12) is effective in treating systemic microscopic malignancies by inducing T helper 1 (T(H)1) response, inhibiting angiogenesis, and triggering secondary cytokine production. Unfortunately, daily systemic administration of an acute dose of IL-12 protein is very costly and severely...
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PMID: 18370211
PDF is available here.
PDF is available here.
Abstract
Electroporation is a unique system for drug and gene delivery, as it is possible to very specifically target certain tissues within the body with whatever drug, gene, isotope, or other product is desired in a specific situation. An increasing number of clinical trials are being launched, and sophist...
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PMID: 18370213
PDF is available here.
PDF is available here.
Abstract
Several studies of DNA vaccination against HER2/neu showed the effectiveness of immunization protocols in models of transplantable or spontaneous tumors. The DNA delivery system plays a crucial role in the success of DNA vaccination. In particular, our studies of DNA vaccination against HER2/neu tum...
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PMID: 18370223
PDF is available here.
PDF is available here.
Abstract
We are presently aware of two early-phase DNA vaccine clinical trials in humans using electroporation-enhanced vaccine delivery. Moreover, two phase I immunogenetherapy studies are in progress and several tolerability studies have been performed on healthy volunteers. We have used knowledge from the...
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PMID: 18370225
PDF is available here.
PDF is available here.
Abstract
We outline the technique of intramuscular electroporation of HGF gene as a remedy for hypertension....
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PMID: 18370217
PDF is available here.
PDF is available here.
Abstract
Electroporation is a powerful method for gene delivery to dystrophic muscle in the mdx mouse model of Duchenne muscular dystrophy. Successful transfer of reporter and therapeutic plasmids and antisense oligonucleotides has been demonstrated. However, the efficiency falls with increasing plasmid size...
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PMID: 18370219
PDF is available here.
PDF is available here.
Abstract
Non-viral gene transfer into skeletal muscle in vivo is enhanced by electroporation (EP) to efficiencies far beyond any other (non-EP) method reported to date. Electroporation consistently delivers high levels of transgene to muscle and has been used extensively for the delivery of therapeutic trans...
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PMID: 18370218
PDF is available here.
PDF is available here.