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Organoids (0):
Recent article
Organoids
Abstract
We transitioned the technique to a mouse model, allowing investigation of the processes involved during TESI formation through the transgenic tools available in this species. This is a necessary step toward applying the technique to human therapy. Multicellular organoid units were derived from small...
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PMID: 21395443
PDF is available here.
PDF is available here.
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Abstract
We found that field stimulation using symmetric biphasic square pulses at 2.5 V/cm, 1 Hz, 1 ms (per pulse phase) was an improved stimulation protocol, as compared to no stimulation and stimulation using monophasic square pulses of identical total amplitude and duration (5 V/cm, 1 Hz, 2 m...
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PMID: 18783322
PDF is available here.
PDF is available here.
Abstract
Our results suggest that morphological cues initiate the differentiation of early hepatic precursors and confirm the inhibitory role of TGF-beta on hepatocytic lineage differentiation.
(c) 2010 Wiley-Liss, Inc....
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PMID: 20458741
PDF is available here.
PDF is available here.
Abstract
We have shown that PLDs (phospholipases D) and their product, PA (phosphatidic acid), belong to the regulators of the secretory pathway in plants. We have also shown that specific NOXs (NADPH oxidases) producing ROS (reactive oxygen species) are involved in cell growth as exemplified by pollen tubes...
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PMID: 20298250
PDF is available here.
PDF is available here.
Methods in Molecular Biology (633)633 2010
Abstract
Limited understanding of the cell biology of the breast and breast cancer hampers our ability to develop new therapeutic approaches. Mouse models of mammary gland development and tumourigenesis are key to developing new insights into the biology of both the normal and diseased tissues. Recent advanc...
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PMID: 20204626
PDF is available here.
PDF is available here.
Cell Transplantation (19)6 2010
Abstract
We show that hepatocytes migrated toward and adhered and formed sinusoids-like structures in conjunction with liver nonparenchymal cells, and that this liver organoid formed sophisticated tissue after 7 days in an implanted LAD in rodents. Hepatocytes only or in combination with human nonparenchymal...
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PMID: 20573303
PDF is available here.
PDF is available here.
Parasitology (85 (Pt 3))03 2009
Abstract
Six murine monoclonal antibodies to Leishmania tropica major have been prepared and the properties of these antibodies studied. Two (WIC 79.3 and 79.7) were L. tropica major species-specific and bound to promastigote cell surfaces, to parasitized macrophages, but not isolated amastigotes. No evidenc...
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PMID: 6184664
PDF is available here.
PDF is available here.
Journal of Surgical Research (156)2 2009
Abstract
Autologous engineered small intestine and stomach formed. Tissue-engineered intestinal architecture replicated that of native intestine. Histology revealed tissue-engineered small intestinal mucosa composed of a columnar epithelium with all differentiated intestinal cell types adjacent to an innerva...
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PMID: 19665143
PDF is available here.
PDF is available here.
Parasitology (89 ( Pt 1))01 2009
Abstract
The ultrastructure of the tegument of Schistosoma haematobium was examined using scanning and transmission electron microscopy. The surface of the male worm is characterized by numerous raised tubercles bearing apically directed spines. The female in contrast to the male is cylindrical and relativel...
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PMID: 6472886
PDF is available here.
PDF is available here.
Biomacromolecules (10)7 2009
Abstract
We analyze the applicability of a novel, photosensitive hydrogel (Lutrol) for printing of 3D structured bone grafts. We benefit from the fast temperature-responsive gelation ability of thermosensitive Lutrol-F127, ensuring organized 3D extrusion, and the additional stability provided by covalent pho...
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PMID: 19445533
PDF is available here.
PDF is available here.
Abstract
We identified PRA-regulated genes by microarray analysis of cultured epithelial organoids derived from pubertal and adult mouse mammary glands, developmental stages with differing progesterone responsiveness. Microarray analysis showed significant progestin (R5020)-regulation of 162 genes in puberta...
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PMID: 19383543
PDF is available here.
PDF is available here.
Abstract
We hypothesized that cultivation of cardiomyocytes (CM) on preformed networks of fibroblasts (FB) and endothelial cells (EC) would enhance the structural and functional properties of the organoids, compared to simultaneously seeding the three cell types or cultivating enriched CM alone. Microchannel...
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PMID: 18442120
PDF is available here.
PDF is available here.
Parasitology (94 ( Pt 2))02 2009
Abstract
The long-term, in vivo effects of a single, subcurative dose (200 mg/kg body weight of mouse) of praziquantel on the structure of adult Schistosoma mansoni and on the process and speed of tegumental repair are described. In both male and female worms praziquantel caused often extensive damage to the...
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PMID: 3108831
PDF is available here.
PDF is available here.
Parasitology (92 ( Pt 1))01 2009
Abstract
Computer-aided reconstruction from serial sections has been used to analyse the 3-dimensional structure of entire amastigotes of Leishmania mexicana and to determine the number, arrangement and volume of each organelle. In two reconstructions, the lysosome-like 'megasomes' were the most numerous org...
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PMID: 3754324
PDF is available here.
PDF is available here.
EMBO Journal (27)21 2008
Abstract
We define the processes by which mammary epithelial cells integrate biochemical and mechanical extracellular cues to maintain their differentiated phenotype. We used single cells cultured on top of gels in conditions permissive for beta-casein expression using atomic force microscopy to measure the...
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PMID: 18843297
PDF is available here.
PDF is available here.
Abstract
The RWV allowed development of columnar epithelium-like structures. Higher magnification revealed well-developed brush borders at the apical side of the tissue. Incubation with C. parvum resulted in patchy disruption of the epithelium and, at the surface of several epithelial cells, in localized inf...
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PMID: 18498239
PDF is available here.
PDF is available here.
Transplantation (85)11 2008
Abstract
We report a novel EPO-producing organ-like structure (organoid) derived from human mesenchymal stem cells. Using our previously established relay culture system, a human mesenchymal stem cell-derived, human EPO-competent organoid was established in rat omentum. The organoid-derived levels of human E...
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PMID: 18551074
PDF is available here.
PDF is available here.
Cancer Research (68)12 2008
Neo-organoid of marrow mesenchymal stromal cells secreting interleukin-12 for breast cancer therapy.
Abstract
We hypothesized that MSCs, gene enhanced to express interleukin-12 (IL-12) and then embedded in a matrix, may act as an anticancer neo-organoid when delivered s.c. in autologous/syngeneic hosts. We performed such experiments in mice and noted that primary murine MSCs retrovirally engineered to secre...
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PMID: 18559528
PDF is available here.
PDF is available here.
Abstract
Both cell growth and function were excellent after harvesting on E35. The pulsed administration of a high concentration of HGF promoted the differentiation and maturation of these fetal hepatic cells. Microscopic examination of organoids in the RFB revealed palisading and showed that bile duct-like...
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PMID: 18461659
PDF is available here.
PDF is available here.
Abstract
We examined whether SHs could express hepatic transporters with polarity, whether the transporters could transport organic anion substrates into BC, and whether the secreted substances could be recovered from BC. Immunocytochemistry and RT-PCR were carried out. [(3)H]-labeled estrogen derivatives we...
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PMID: 18022823
PDF is available here.
PDF is available here.
Endocrinology (149)5 2008
Abstract
We investigated the effects of HGF and/or R5020 on morphology and LEC- and MEC-specific in vitro proliferation in organoids. HGF-induced tubulogenesis was initiated and carried out by LECs starting with cellular extensions, followed by the formation of chains and cords, and culminating in tubule for...
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PMID: 18218689
PDF is available here.
PDF is available here.
Differentiation (76)5 2008
Abstract
We show that expression of R522K-Shb results in up-regulation of FGFR1 and FGF2 in EBs. Addition of activin A to the cultures enhances the expression of endodermal genes primarily in EBs expressing mutant Shb. Inhibition of FGF signaling by the addition of the FGFR1 inhibitor SU5402 completely count...
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PMID: 18093225
PDF is available here.
PDF is available here.
Abstract
We exploit the self-organizing capacity of cells and tissues to construct functional living structures of prescribed shape. In our technology, multicellular spheroids (bio-ink particles) are placed into biocompatible environment (bio-paper) by the use of a three-dimensional delivery device (bio-prin...
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PMID: 18333793
PDF is available here.
PDF is available here.
Artificial Organs (32)3 2008
Abstract
We have proposed a tissue-engineered stomach as a replacement of the native stomach. This study aimed to assess the secretory functions of a tissue-engineered stomach in a rat model and the nutritional status of the recipients over an extended time period. Stomach epithelial organoid units were isol...
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PMID: 18201286
PDF is available here.
PDF is available here.
Abstract
We have developed scaffold-free culture methods in which the cells form multicellular aggregates (organoids). In this study, we applied the organoid culture method to chondrocytes attempting to reconstitute articular cartilage grafts. Primary rat costal chondrocytes and subcultured human articular c...
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PMID: 18374148
PDF is available here.
PDF is available here.
Abstract
We introduce fully biological, living engineered cardiac organoids, or simplified heart chambers, that beat spontaneously, develop pressure, eject fluid, contain residual stress, exhibit a functional Frank-Starling mechanism, and generate positive stroke work. We also demonstrate regional variations...
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PMID: 18333774
PDF is available here.
PDF is available here.
Experimental Cell Research (314)3 2008
Abstract
We obtained early EPCs from human peripheral blood and late EPCs from umbilical cord blood. Human umbilical vascular endothelial cells (HUVECs) were also used. Cells were evaluated for their tube-forming potential using our novel in vitro assay system. Cells were seeded linearly along a 60 mum wide...
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PMID: 18083163
PDF is available here.
PDF is available here.
Abstract
Cells in tissues do not exist as isolated entities but are part of the three-dimensional tissue architecture. Consequently, some aspects of cell behaviour cannot be mimicked by simple in vitro monolayer culture systems. Moreover, cell shape and behaviour is not rigid but is dynamic and can be regula...
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PMID: 19099262
PDF is available here.
PDF is available here.
Abstract
We studied the recovery of rats with fulminant hepatic failure (FHF) by treating them with our original hybrid artificial liver support system (HALSS). We developed an original artificial liver module having a liver lobule-like structure (LLS). This module consists of many hollow fibers regularly ar...
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PMID: 18286455
PDF is available here.
PDF is available here.
Abstract
Optimized medium composition allows nearly all seeded RWPE-1 cells to undergo acinar morphogenesis, forming consistent structures representative of normal adult prostate glands. Low-calcium-containing medium appears selective for cells capable of undergoing acinar morphogenesis in vitro, and branchi...
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PMID: 17705248
PDF is available here.
PDF is available here.
Cancer Research (67)17 2007
Abstract
We have isolated a population of mouse mammary gland cells that are capable of forming "mammospheres" in vitro. Importantly, mammospheres are enriched for cells that regenerate an entire mammary gland on implantation into a mammary fat pad. We also undertook cytogenetic analyses of mammosphere-formi...
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PMID: 17804725
PDF is available here.
PDF is available here.
Journal of Membrane Biology (217)1-3 2007
Abstract
We examined the possible relationship between functional intact gap junctional intercellular communication (GJIC) and fenestrae dynamics in M1-SEC cells. The fine morphology of liver organoid was studied in the presence of (1) irsogladine maleate (IM), (2) oleamide and (3) oleamide followed by IM tr...
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PMID: 17568973
PDF is available here.
PDF is available here.
Abstract
We report that thymic stroma, arrayed on a synthetic 3D matrix, supports the development of functional human T cells from hematopoietic precursor cells. Newly generated T cells contain T-cell receptor excision circles and are both fully mature and functional. The coculture of T-cell progenitors with...
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PMID: 17876092
PDF is available here.
PDF is available here.
Abstract
We found that the geometry of tubules dictates the position of branches. We predicted numerically and confirm experimentally that branches initiate at sites with a local minimum in the concentration of autocrine inhibitory morphogens, such as transforming growth factor-beta. These results reveal tha...
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PMID: 17038622
PDF is available here.
PDF is available here.
Artificial Organs (30)10 2006
Abstract
Extracorporeal human lymphatic organs are expected to be excellent tools in the study of human molecular and cellular bases of the immunologic balance and tissue harmony. A rational approach and process to design a device and a procedure to recreate the human lymph node environment in vitro is descr...
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PMID: 17026580
PDF is available here.
PDF is available here.
Artificial Organs (30)10 2006
Abstract
Progresses in polymeric membrane preparation and in the understanding and control of their transport properties make possible the design of novel membranes to be used for cell culture (e.g., hepatocytes, lymphocytes, pancreatic islets) in biohybrid systems such as therapeutic device or as in vitro m...
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PMID: 17026579
PDF is available here.
PDF is available here.
Abstract
Orthotopic transplantation of epithelial organoids containing ileal stem cells was used to generate a neoileal mucosa that expressed all 4 intestinal lineages along with a new zone of active bile acid uptake and IBAT expression in a recipient jejunal segment....
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PMID: 16934605
PDF is available here.
PDF is available here.
Abstract
Abstract
We have adopted a biomimetic approach and assembled microscale modular components, consisting of submillimeter-sized collagen gel rods seeded with endothelial cells (ECs) into a (micro)vascularized tissue; in some prototypes the gel contained HepG2 cells to illustrate the possibilities. The EC-cover...
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PMID: 16864785
PDF is available here.
PDF is available here.
Journal of Surgical Research (134)1 2006
Abstract
This novel system allows continuous in vivo observation of engrafted cells that are seeded on externalized omentum. The use of PGA mesh biopolymer may improve engraftment of intestinal organoids....
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PMID: 16697415
PDF is available here.
PDF is available here.
Abstract
Our aim was to establish and characterize biologically meaningful 3-D models of human colonic epithelium and apply them to study the early stages of enteric salmonellosis. The human colonic cell line HT-29 was cultured in 3-D and characterized by immunohistochemistry, histology, and scanning electro...
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PMID: 16730210
PDF is available here.
PDF is available here.
Molecular Therapy (13)6 2006
Abstract
We investigated a novel nonwoven fibrous scaffold as a vehicle for delivery of DNA. Fibers were formed by polyelectrolyte complexation of water-soluble chitin and alginate, and PEI-DNA nanoparticles were encapsulated during the fiber drawing process. Nanoparticles released from the fibers over time...
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PMID: 16497560
PDF is available here.
PDF is available here.
Abstract
Three-dimensional, vascularized skeletal muscle can be engineered in vivo. The resulting tissues have histologic and functional properties consistent with native skeletal muscle....
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PMID: 16772923
PDF is available here.
PDF is available here.
Circulation (113)18 2006
Abstract
ES cells can be used as a source of seed cells for cardiac tissue engineering. Additional work remains to demonstrate engraftment of the engineered heart tissue in the case of cardiac defects and its functional integrity within the host's remaining healthy cardiac tissue....
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PMID: 16651472
PDF is available here.
PDF is available here.
Abstract
By transmission electron microscopy, FLC-5, M1, and A7 were arranged in relation to the perfusion side in a liver-like organization. Structures resembling bile canaliculi were seen between FCL-5 cells. Scanning electron microscopy demonstrated fenestrae on SEC surfaces. The number of vesiculo-vacuol...
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PMID: 16609994
PDF is available here.
PDF is available here.
Abstract
In recent years, use of hepatocyte aggregates has led to development of a hybrid artificial liver support system (HALSS) that has high performance. However, in general, their thickness is 100 microm or more, and generation of a dead cell layer due to oxygen exhaustion inside the aggregates has been...
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PMID: 16685676
PDF is available here.
PDF is available here.
Abstract
Our novel approach is to implant tubular poly-lactide-co-glycolide (PGLA) foam scaffolds subcutaneously. The aim of this study was to investigate whether these scaffolds would support growth of intestinal neomucosa. PGLA scaffolds were implanted subcutaneously into 8 Lewis rats; after 5 weeks, 'orga...
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PMID: 16447140
PDF is available here.
PDF is available here.
Cell Transplantation (15 Suppl 1) 2006
Abstract
The availability of new cell culture, cell transplantation, and gene expression technology has highlighted a role of hepatocytes as facultative stem cells for the biliary epithelium. In addition, hepatocytes can revert to primitive progenitor cell types and express gene profiles that appear as chara...
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PMID: 16826797
PDF is available here.
PDF is available here.
Biomaterials (27)3 2006
Abstract
We cultured human NP cell line HNPSV-1, in three different Atelocollagen and compared with alginate. Furthermore, NP-like tissues were generated using these cells and different Atellocollagen solutions. Results showed that both DNA synthesis and content is significantly greater when cultured in Atel...
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PMID: 16102816
PDF is available here.
PDF is available here.
Infection and Immunity (74)1 2006
Abstract
We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium....
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PMID: 16369034
PDF is available here.
PDF is available here.
Development (132)17 2005
Abstract
We examined the role of each of these molecules in mammary development. Tissue recombination and transplantation studies revealed that EGFR phosphorylation and ductal development occur only when ADAM17 and AREG are expressed on mammary epithelial cells, whereas EGFR is required stromally, and that l...
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PMID: 16079154
PDF is available here.
PDF is available here.
FASEB Journal (19)11 2005
Abstract
We have shown that when an arterio-venous (AV) loop is constructed in vivo in the rat groin, and placed inside a semisealed chamber, an extensive functional vasculature is generated. To test whether this unusually angiogenic environment supports the survival and growth of implanted tissue or cells,...
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PMID: 16014398
PDF is available here.
PDF is available here.
Abstract
All germ cells throughout the animal kingdom contain cytoplasmic cloud-like accumulations of material called nuage. Polar bodies in Drosophila oocytes are probably the best known forms of nuage. In spermatogenic cells, the nuage is called chromatoid body (CB). In early spermatids of the rat, it has...
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PMID: 16048630
PDF is available here.
PDF is available here.
Journal of Cell Science (118)Pt 12 2005
Abstract
Maintenance of epithelial cell shape and polarity determines many vital cell functions, including the appropriate response to external stimuli. Murine hepatocytes cultured in a three-dimensional Matrigel matrix formed highly polarized organoids characterized by specific localization of an ERM (ezrin...
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PMID: 15944402
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to investigate the in vitro response of human craniofacial muscle-derived myotubes (primitive/nascent muscle fibres), in three-dimensional constructs, to strain in vitro to mimic clinical scenarios, using expression of the mechanoresponsive gene gelatinase-A/matrix metallop...
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PMID: 15953246
PDF is available here.
PDF is available here.
Abstract
SH could differentiate into mature hepatocytes by the addition of Matrigel and re-express constitutive CYPs. The expression of CYP1A1/2, CYP2B1, CYP3A2, and CYP4A1 dose-dependently increased and the amounts gradually increased with time in culture, especially in the cells treated with Matrigel. Acti...
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PMID: 15946133
PDF is available here.
PDF is available here.
Abstract
We investigated the mechanism of phenotypic plasticity of hepatocytes in a three-dimensional organoid culture system, in which hepatocytic spheroids were embedded within a collagen gel matrix. Hepatocytes expressed several bile duct markers including cytokeratin (CK) 19 soon after culture and underw...
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PMID: 15793288
PDF is available here.
PDF is available here.
Laryngoscope (115)2 2005
Abstract
Human salivary gland epithelial cells retained their phenotypic and functional characteristics at all culture stages. Histologically, formation of acinar gland-like structures was observed within the engineered tissue by 4 weeks after implantation. Immunocytochemical and Western blot analyses of the...
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PMID: 15689743
PDF is available here.
PDF is available here.
Journal of Cell Science (118)Pt 3 2005
Abstract
We show that premalignant mammary epithelial cells exposed to senescent human fibroblasts in mice irreversibly lose differentiated properties, become invasive and undergo full malignant transformation. Moreover, using cultured mouse or human fibroblasts and non-malignant breast epithelial cells, we...
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PMID: 15657080
PDF is available here.
PDF is available here.
Nature Biotechnology (22)12 2004
Abstract
We demonstrate that a tissue-engineered, lymphoid tissue-like organoid, which was constructed by transplantation of stromal cells embedded in biocompatible scaffolds into the renal subcapsular space in mice, had an organized tissue structure similar to secondary lymphoid organs. This organoid contai...
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PMID: 15568019
PDF is available here.
PDF is available here.