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Defective Viruses (5):
Recent article
Defective Viruses
Cold Spring Harbor Protocols (2011)5 2011
Abstract
Engineering effective vectors has been crucial to the efficient delivery and expression of therapeutic gene products in vivo. Among these, HSV-1 represents an excellent candidate vector for delivery to the peripheral and central nervous systems. The natural biology of HSV-1 includes the establishmen...
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PMID: 21536761
PDF is available here.
PDF is available here.
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Abstract
We were able to identify viral RNA molecules which specifically associated with RIG-I during infection. We showed that in Sendai virus infected cells, RIG-I specifically and preferentially associated with the copy-back defective interfering (DI) particle RNA and not with the full-length Sendai virus...
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PMID: 21422808
PDF is available here.
PDF is available here.
Abstract
Hepatitis Delta virus is a defective RNA virus that requires HBV as helper virus. The two viruses share the same route of transmission, being prevalently transmitted by contaminated blood and body fluids. HDV can infect simultaneously with HBV (coinfection) or in a patient with already established H...
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PMID: 20433000
PDF is available here.
PDF is available here.
Abstract
We describe here the construction and characterization of deletion mutants defective in the glycoprotein E (gE) or thymidine kinase (TK) gene or both (gE/TK) from a highly neurovirulent and well-characterized Brazilian BoHV-5 strain (SV507/99). A gE-deleted recombinant virus (BoHV-5 gE) was first ge...
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PMID: 19893987
PDF is available here.
PDF is available here.
Abstract
Vaginal disorders associated with systemic chemotherapy arise by direct inhibition of the resident microbiota (dominated by lactobacilli) or, possibly, by induction of prophages harbored in their genomes, leading to cell lysis. In the present study, proficient Lactobacillus phages could not be isola...
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PMID: 19784933
PDF is available here.
PDF is available here.
Abstract
We used an integrase-defective lentiviral (IDLV) vector to decrease the chance of random integration and examined the feasibility of lentiviral vector-mediated gene targeting into murine embryonic stem (ES) cells. After transduction with wild-type lentiviral vectors, none of the 512 G418 resistant c...
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PMID: 19208434
PDF is available here.
PDF is available here.
Journal of General Virology (90)Pt 4 2009
Abstract
Our analysis supports a multiple-hit process where DIPs inhibit or promote virus and DIP production, depending on dose. Specifically, three regimes of co-infection were apparent: (i) low DIP - where both virus and DIPs are amplified, (ii) medium DIP - where amplification of both virus and DIPs is in...
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PMID: 19264636
PDF is available here.
PDF is available here.
Abstract
Gene therapy with hematopoietic stem cells (HSC) is an attractive therapeutic strategy for several forms of primary immunodeficiencies. Current approaches are based on ex vivo gene transfer of the therapeutic gene into autologous HSC by vector-mediated gene transfer. In the past decade, substantial...
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PMID: 20008254
PDF is available here.
PDF is available here.
Abstract
Hematopoietic stem cell (HSC)-targeted gene transfer is an attractive approach for the treatment of a number of hematopoietic disorders caused by single gene defects. Indeed, in a series of gene transfer trials for two different primary immunodeficiencies beginning early in this decade, outstanding...
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PMID: 20008255
PDF is available here.
PDF is available here.
Abstract
We describe a method for studying persistent infection using recombinant DIP (rDIP). Infection with rDIP resulted in the production of recombinant DIP that replicated faithfully to, at least, five passages and mediated a rapid progression to persistent infection as measured by: 1) production of cell...
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PMID: 18805562
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)17 2008
Abstract
Two independent isolates of the gut commensal Lactobacillus johnsonii were sequenced. These isolates belonged to the same clonal lineage and differed mainly by a 40.8-kb prophage, LJ771, belonging to the Sfi11 phage lineage. LJ771 shares close DNA sequence identity with Lactobacillus gasseri prophag...
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PMID: 18515417
PDF is available here.
PDF is available here.
Journal of Virology (82)17 2008
Abstract
We describe a new concept in antivirals based on a defined, naturally occurring defective influenza virus RNA that has the potential to protect against any influenza A virus in any animal host. This "protecting RNA" (244 RNA) is incorporated into virions which, although noninfectious, deliver the RN...
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PMID: 18579602
PDF is available here.
PDF is available here.
Journal of Virology (82)17 2008
Abstract
We developed a novel HSV vaccine strategy that uses a replication-competent strain of HSV-1, NS-gEnull, which has a defect in anterograde and retrograde directional spread and cell-to-cell spread. Following scratch inoculation on the mouse flank, NS-gEnull replicated at the site of inoculation witho...
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PMID: 18562543
PDF is available here.
PDF is available here.
Abstract
Our data indicate that an elevated dHBV/HBV ratio is associated with liver necroinflammation and fibrosis disease, suggesting a regulation of dHBV expression according to the severity of the liver disease. The dHBV/HBV ratio may help to better define liver disease stage during HBV infection....
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PMID: 18532883
PDF is available here.
PDF is available here.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (39)4 2008
Abstract
By sequencing, it was confirmed that the product was the gene of hNET. PCR test, restriction endonuclease digestion and Western Blotting confirmed the successful construction of the recombinants Ad-hNET. The titre of purified recombinant adenovirus Ad-hNET was 1.2 X 10(10) pfu/mL. CONCLUSION: The re...
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PMID: 18798484
PDF is available here.
PDF is available here.
Avian Diseases (52)2 2008
Abstract
The avian adeno-associated virus (AAAV) is a replication-defective nonpathogenic virus member of the family Parvoviridae that has been proved to be useful as a viral vector for gene delivery. The use of AAAV for transgenic expression of Newcastle disease virus (NDV) hemagglutinin-neuraminidase (HN)...
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PMID: 18646454
PDF is available here.
PDF is available here.
Journal of Biotechnology (134)3-4 2008
Abstract
We aim to produce a safe disabled HVS-based recombinant viral system for gene therapy applications. An HVS recombinant viral amplicon was constructed with a transgene packaging potential of 50 kb. The recombinant HVS genome was shown to be replication disabled and used to generate a stable cell line...
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PMID: 18328588
PDF is available here.
PDF is available here.
Journal of Immunology (180)7 2008
Abstract
We show that 1) DI particles are recognized by more than one viral sensor independently of TLRs and type I IFN signaling; 2) The helicase MDA5 participates in the detection of DI genomes as MDA5-deficient DCs respond inefficiently to Sendai virus stocks containing DI particles; 3) DI particles stimu...
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PMID: 18354215
PDF is available here.
PDF is available here.
Abstract
We disrupted its U(L)41 gene, producing the triple mutant virus dl5-29-41L. dl5-29-41L has a decreased ability to inhibit host cell protein synthesis and a reduced cytopathic effect on cultured cells. When used to immunize mice, dl5-29-41L elicited significantly stronger neutralizing antibody respon...
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PMID: 18006033
PDF is available here.
PDF is available here.
Abstract
I and then co-transformed respectively with the adenoviral genome vector pAdEasy-1 into E. coli BJ5183. The successful recombinants were selected by Kanamycin and confirmed by Pac I digestion. The recombinant vectors Ad-hSef-L-Myc and Ad-hSef-S-Myc were finally digested with Pac I and transfected in...
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PMID: 18464599
PDF is available here.
PDF is available here.
Molecular Cell (29)1 2008
Abstract
We demonstrate that both ongoing transcription and pre-mRNA processing are essential for gene loop formation, and predict that these structures represent a defining feature of active gene transcription....
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PMID: 18206969
PDF is available here.
PDF is available here.
Abstract
We employed reporter viruses and quantitative real time PCR to examine gene expression and virus replication during coinfection with integrating and non-integrating HIV-1. Most cells which contained only uDNA displayed no detected expression from fluorescent reporter genes inserted into early (Rev-i...
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PMID: 18613957
PDF is available here.
PDF is available here.
Abstract
I and II clinical trials in prostate cancer. Enhanced cell killing by apparent immune-mediated mechanisms has been shown in pancreatic and colorectal cancer models, by co-expressing murine granulocyte macrophage colony-stimulating factor (GM-CSF) with NR in a single replication deficient adenoviral...
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PMID: 17726452
PDF is available here.
PDF is available here.
Archives of Virology (153)9 2008
Abstract
Our data prove that BBMV DI RNAs, whose yield is similar to the virus genomic components, are useful as gene vectors after mechanical inoculation on legume plants....
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PMID: 18679766
PDF is available here.
PDF is available here.
Abstract
We describe the current state of these first- and second-generation vaccine candidates, and compare these products to our recently described single-cycle, encapsidation defective flavivirus vaccine: RepliVAX. RepliVAX can be propagated in C-expressing cells (or as a unique two-component virus) using...
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PMID: 19081489
PDF is available here.
PDF is available here.
Abstract
Lentivectors have drawn considerable attention recently and become important delivery vehicles for gene transfer manipulation. By Transiently co-transfecting 293T packaging cells with three DNA plasmids system encoding lentivector constituents, a protocol for bulky preparation of human immunodeficie...
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PMID: 18271264
PDF is available here.
PDF is available here.
Abstract
We hypothesize that cluster formation and sustaining infected cell percentage increase the likelihood of co-infection by a DI and wt RUB during serial passage thus enhancing maintenance of the DI. Cluster formation and sustaining infected cell percentage were found to be due to a combination of atte...
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PMID: 17698161
PDF is available here.
PDF is available here.
Abstract
A single-cycle simian immunodeficiency virus (scSIV) that undergoes only one round of infection and replication was constructed to calculate the total number of virons produced by an SIV-infected cell in vivo. Four Mamu-A*01 rhesus macaques were inoculated on two occasions 11 weeks apart with the sc...
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PMID: 18025463
PDF is available here.
PDF is available here.
Abstract
We tested the ability of an HSV-2 replication-defective virus, a genital herpes vaccine candidate, to protect against HSV-1 corneal infection. Immunization with HSV-2 dl5-29 reduced viral replication in the cornea, prevented ocular disease and reduced latent infection by the HSV-1 strain. Therefore,...
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PMID: 17915278
PDF is available here.
PDF is available here.
Current protocols in microbiology (Chapter 16) 2007
Abstract
This unit describes principles and protocols for expressing a gene of interest in plant cells using gene vectors that are derived from an infectious full-length cDNA plasmid of the tomato bushy stunt virus (TBSV) genomic RNA, and from defective interfering RNAs (DIs). The TBSV gene vector system per...
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PMID: 18770620
PDF is available here.
PDF is available here.
Physiological Genomics (31)2 2007
Abstract
Lentiviral vectors have become a promising new tool for the establishment of transgenic animals and the manipulation of the mammalian genome. While conventional microinjection-based methods for transgenesis have been successful in generating small and large transgenic animals, their relatively low t...
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PMID: 17684037
PDF is available here.
PDF is available here.
Abstract
We previously developed a bipartite TMV-defective RNA (dRNA) system to dissect the role of the N-terminal methyltransferase (MT) domain in accumulation and cell-to-cell movement of dRNAs [Knapp, E., Danyluk, G.M., Achor, D., Lewandowski, D.J., 2005. A bipartite Tobacco mosaic virus-defective RNA (dR...
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PMID: 17602721
PDF is available here.
PDF is available here.
Journal of General Virology (88)Pt 10 2007
Abstract
We characterized a persistently Junín virus (JUNV)-infected BHK-21 cell line obtained by experimental infection with the XJCl3 strain. This cell line, named K3, produced low levels of virus in supernatants which were not influenced by the presence of defective interfering (DI) particles after the f...
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PMID: 17872526
PDF is available here.
PDF is available here.
Chinese Medical Journal (120)18 2007
Abstract
Comet-like adenovirus-producing foci of Ad-GFP/tk and Ad-GFP were observed after 5 to 7 days of cell culture. After twelve days of packaging, the replication-defective adenoviruses were collected. Identified with PCR, thymidine kinase gene was successfully constructed into Ad-GFP/tk. CONCLUSION: The...
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PMID: 17908483
PDF is available here.
PDF is available here.
Cancer Gene Therapy (14)9 2007
Abstract
We tested the activity of two non-replicative herpes simplex virus (HSV)-1-based vectors, encoding human endostatin::angiostatin or endostatin::kringle5 fusion proteins in combination with HSV-1 thymidine kinase (TK) molecule, on endothelial cells (ECs) and Lewis lung carcinoma (LLC) cells. We obser...
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PMID: 17557110
PDF is available here.
PDF is available here.
Abstract
We add to recent reports indicating that individual variance occurs due to the integration of non-retroviral (potyviral) RNAs into the host genome via RNA recombination followed by retrotransposition. We report that in bees (Apis mellifera), approximately 30% of all tested populations carry a segmen...
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PMID: 17275871
PDF is available here.
PDF is available here.
Molecular Biotechnology (36)1 2007
Abstract
We previously described that Sabin3-like mutation introduced in Coxsackievirus B3 (CVB3) genome led to a defective mutant. However, this mutation do not led to destruction of secondary structure motif C within the stem-loop V of CVB3 RNA because of the presence of one nucleotide difference (C --> U)...
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PMID: 17827538
PDF is available here.
PDF is available here.
Zhongguo Xiufu Chongjian Waike Zazhi/Chinese Journal of Reparative and Reconstructive Surgery (21)4 2007
Abstract
The pAdE/hBMP-4 was confirmed by the restrictional endonuclease digestion. In HEK293 and HeLa cells, the specific transcription of the hBMP-4 gene was confirmed by RT-PCR, and the expression of the hBMP-4 protein was confirmed by the Western-blot assay. CONCLUSION: The replication-defective recombin...
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PMID: 17546887
PDF is available here.
PDF is available here.
Abstract
We have optimized lentiviral-based gene transfer for cultured motoneurons by using a replication-defective viral vector system. These techniques result in transduction efficacies higher than 50%, as judged by EGFP expression under the control of SFFV or CMV promoters. Under the same conditions, surv...
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PMID: 17102992
PDF is available here.
PDF is available here.
Journal of Virology (81)8 2007
Abstract
We have examined the efficiencies of reverse transcription and integration and find that approximately 1 of every 8 virions that initiate reverse transcription form proviruses, a quantity significantly different from the commonly reported ratio of 1 in 1,000. In addition, results from two different...
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PMID: 17267494
PDF is available here.
PDF is available here.
Abstract
Two lysis-defective but DNA synthesis non-defective temperature-sensitive (ts) mutants of mycobacteriophage L1, L1G23ts23 and L1G25ts889 were found to be defective also in phage-specific RNA synthesis in the late period of their growth at 42 degrees C, each to the extent of 50% of that at 32 degrees...
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PMID: 17394764
PDF is available here.
PDF is available here.
Abstract
We immunized mice orally and systemically with a chimpanzee derived adenoviral vector expressing HIV gag (AdC68gag) and measured frequencies of gag-specific interferon-gamma (IFN-gamma) producing CD8(+) T cells in the GALT. A single oral administration was inefficient at eliciting responses in the m...
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PMID: 17229501
PDF is available here.
PDF is available here.
Virus Research (124)1-2 2007
Abstract
We have identified for the first time the generation of DI RNAs of Japanese encephalitis virus in C6/36 mosquito cells. A persistent infection was established by replacing growth medium on surviving cells and continued cell passaging. Persistent infection was demonstrated by a continual release of i...
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PMID: 17134784
PDF is available here.
PDF is available here.
Journal of Virology (81)5 2007
Abstract
We used a sensitive coculture assay in an attempt to isolate replication-competent virus from a cohort of 10 ES. We successfully cultured six replication-competent isolates from 4 of the 10 ES. The frequency of latently infected cells in these patients was more than a log lower than that seen in pat...
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PMID: 17151109
PDF is available here.
PDF is available here.
Trends in Microbiology (15)2 2007
Abstract
We use the genomic sequence data that are currently available to identify candidate GTA-producing species and propose an evolutionary scheme for RcGTA-like elements in the alpha-proteobacteria....
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PMID: 17184993
PDF is available here.
PDF is available here.
Abstract
We evaluated the properties of a new generation HSV vaccine vector, an HSV-1 multiple immediate-early (IE) gene deletion mutant virus, d106, which contains deletions in the ICP4, ICP27, ICP22, and ICP47 genes. Because several of the HSV IE genes have been implicated in immune evasion, inactivation o...
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PMID: 16996101
PDF is available here.
PDF is available here.
Abstract
We now report that the addition of either a pancaspase inhibitor or caspase-9-specific inhibitor prevented cells infected with an apoptotic HSV-1 virus from undergoing cell death. This result indicated that HSV-1-dependent apoptosis proceeds through the mitochondrial apoptotic pathway. However, the...
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PMID: 17080326
PDF is available here.
PDF is available here.
Abstract
Modified Vaccinia virus Ankara (MVA) constitutes a good candidate for the development of non-replicative expression viral vectors because it does not replicate in most of mammalian cells. It is essential, for the production of recombinant MVA, the availability of transfer vectors which allow the int...
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PMID: 17987847
PDF is available here.
PDF is available here.
Neuroimmunomodulation (14)5 2007
Abstract
We present here direct evidence that human recombinant viruses can localize in mitochondria to disrupt their integrity. This finding, while opening new perspectives in viral gene therapy, may provide new insights into the pathogenesis, prevention and treatment of viral diseases. In addition, it may...
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PMID: 18219224
PDF is available here.
PDF is available here.
Cancer Gene Therapy (14)1 2007
Abstract
We tested their ability to deliver a transgene, and to target a transcript, so as to inhibit pancreatic tumors growth in vivo. BxPC3 and Capan-1 cells were efficiently transduced using SV40 vectors without selection, as compared to synthetic vectors PEI. SV40 vectors were as efficient as adenoviral...
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PMID: 16990845
PDF is available here.
PDF is available here.
Archives of Virology (152)6 2007
Abstract
Distinct subgenomic DNA species known as defective (df) DNA molecules were found in plants infected with tomato leaf curl virus (TLCV). Four df DNAs derived from TLCV Type and Darwin 1 strains were found to contain large deletions that disrupt all of the viral genes required for viral replication, e...
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PMID: 17347773
PDF is available here.
PDF is available here.
Archives of Virology (152)3 2007
Abstract
Preparations of double-stranded RNAs (dsRNAs) extracted from Nicotiana tabacum cv Xanthi plants infected with a subgroup IB isolate of Cucumber mosaic virus (CMV) were found to contain a heterogeneous population of defective RNAs (D-RNAs) derived from RNA 3. Characterised D-RNAs ranged in size from...
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PMID: 17122894
PDF is available here.
PDF is available here.
Journal of Virology (80)23 2006
Abstract
Our findings emphasize the potential of reverse genetics to improve the efficacy of live influenza vaccines, thus rendering them more suitable for high-risk age groups....
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PMID: 16971432
PDF is available here.
PDF is available here.
Journal of Virology (80)23 2006
Abstract
We report the complete sequence analysis of the provirus harbored in a long-term nonprogressor (patient SG1) 20 years after the first infection with a human immunodeficiency virus type 1 strain lacking nef. The sequencing showed large deletions in the nef-nef and nef-U3 regions. Except for vpu, all...
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PMID: 16987968
PDF is available here.
PDF is available here.
Journal of Virology (80)23 2006
Abstract
We provide evidence that immediate-early protein ICP4 is involved in the process of converting the linear HSV-1 ICP4-deleted mutant strain d120 genome into its endless form. Under conditions where de novo viral DNA synthesis was inhibited, the genome of the ICP4 deletion mutant d120 failed to assume...
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PMID: 16987974
PDF is available here.
PDF is available here.
Journal of Virology (80)23 2006
Abstract
We have examined the function of the ASFV structural protein pB438L, encoded by gene B438L, in virus morphogenesis. We show that protein pB438L associates with membranes during the infection, behaving as an integral membrane protein. Using a recombinant ASFV that inducibly expresses protein pB438L,...
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PMID: 17005638
PDF is available here.
PDF is available here.
Abstract
We showed that the production of viruses containing seven gene segments is very inefficient, which can be overcome by providing the 8th gene segment in a non-functional form. We conclude that sequences in the 5' and 3' ends of the polymerase gene segments contain packaging signals. Our methods will...
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PMID: 16831497
PDF is available here.
PDF is available here.
Abstract
The positive clones were identified with enzyme digestion and polymerase chain reaction (PCR) and were further verified by DNA sequencing. The recombinant adenovirus of high titration was obtained after transfection and packaging in HEK293 cells. CONCLUSION: A recombinant adenovirus vector for expre...
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PMID: 17121697
PDF is available here.
PDF is available here.
Zhonghua Yixue Zazhi (86)40 2006
Abstract
The TGF-beta1 levels of the irradiation groups all increased, peaking in the second week (all P < 0.05), all significantly higher than those of Group C. However, the TGF-beta1 levels at different time points of Group RAR were all significantly lower then those of the other irradiation groups. The CT...
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PMID: 17200022
PDF is available here.
PDF is available here.
Journal of Virology (80)21 2006
Abstract
We have examined the functions of nef alleles from six individuals belonging to the same cohort of hemophiliacs infected with HIV-1 prior to 1985 and classified as LTNP in 1995. Three out of six individuals have progressed to HIV disease (late progressors [LP]), whereas the three remainders have mai...
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PMID: 16943296
PDF is available here.
PDF is available here.