Abstract
Noroviruses (NoVs) are a major etiological agent of sporadic acute gastroenteritis worldwide.
To detect, quantify and characterize genogroups and genotypes of NoVs in children with and without gastrointestinal symptoms.
NoVs were investigated by RT-PCR...
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PMID: 20004146
PDF is available here.
I H M Friesema,
H Vennema,
J C M Heijne,
C M de Jager,
G Morroy,
J H T C van den Kerkhof,
E J M de Coster,
B A Wolters,
H L G ter Waarbeek,
E B Fanoy,
P F M Teunis,
R van der Linde and
Y T H P van Duynhoven
Abstract
Effective infection control measures during norovirus outbreaks are urgently needed in places where vulnerable individuals gather. In the present study, the effect of a number of measures was investigated in daily practice. Forty-nine Dutch nursing homes were monitored prospectively for norovirus ou...
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PMID: 19426572
PDF is available here.
D Werber,
D LauseviÄ,
B Mugosa,
Z Vratnica,
L IvanoviÄ-NikoliÄ,
L ZiziÄ,
A Alexandre-Bird,
L Fiore,
F M Ruggeri,
I Di Bartolo,
A Battistone,
B Gassilloud,
S Perelle,
D Nitzan Kaluski,
M Kivi,
R Andraghetti and
K G J Pollock
Abstract
We estimated the total size of the outbreak to be 10 000-15 000 corresponding to an attack rate of approximately 10%. We conducted an age- and neighbourhood-matched case-control study, microbiologically analysed faecal and municipal water samples and assessed the water distribution system. All cases...
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PMID: 19534843
PDF is available here.
Abstract
Male-specific (F) RNA bacteriophages have been proposed as indicators for human enteric viruses in shellfish. This study compared the use of Escherichia coli and FRNA bacteriophages to indicate the presence and level of noroviruses in Crassostrea gigas. A total of 167 samples from category A and B s...
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PMID: 19903400
PDF is available here.
Abstract
Our objective was to evaluate murine norovirus (MNV-1) and MS2 coliphage (single-stranded RNA) as human enteric virus surrogates for their susceptibility to a novel high-pressure homogenization process for application in commercial settings. Experiments were conducted in duplicate with MNV-1 and MS2...
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PMID: 19903411
PDF is available here.
Abstract
Noroviruses are the most common cause of outbreaks of acute gastroenteritis worldwide. Norovirus outbreaks affect persons of all ages and occur in a wide variety of settings (e.g., nursing homes, hospitals, restaurants, communities, schools, day care centers, military barracks, and cruise ships). Du...
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PMID: 19816397
PDF is available here.
Abstract
We evaluated the norovirus (NoV) detection reagent kit, which uses immunochromatography (IC), against outbreaks of food-borne acute viral gastroenteritis having occurred in Osaka Prefecture between November 2008 and March 2009. A total of 33 outbreaks, RT-PCR identified 27 NoV-positive cases, wherea...
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PMID: 19928492
PDF is available here.
Abstract
In the present study, nanoalumina filters were used as a sample preparation step for the concentration of a norovirus surrogate (murine norovirus 1) from food, and this was coupled with a two-step, real-time reverse transcriptase PCR for quantification. The nanoalumina medium was provided in a syrin...
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PMID: 19833042
PDF is available here.
Abstract
To determine the burden of norovirus infections in children stools from a longitudinal community cohort were evaluated using reverse transcription polymerase chain reaction. Norovirus was detected in 21.3% of diarrheal and 8.0% of nondiarrheal stools (P < 0.01). Norovirus diarrhea was highly associa...
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PMID: 19636281
PDF is available here.
Abstract
Two previously healthy children developed Clostridium difficile ribotype 027-associated disease concomitantly with norovirus infection. Viral gastroenteritis may contribute to epithelial homeostasis of the intestine and exacerbate the effects of toxins produced by C. difficile ribotype 027.
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PMID: 19636284
PDF is available here.
Abstract
We administered a survey to affected staff to evaluate adherence to social distancing recommendations. Of the 102 survey respondents, 74 (73%) completed self-quarantine. Staff adherence was similar regardless of job responsibility. Incomplete adherence to recommendations could potentially accelerate...
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PMID: 19619058
PDF is available here.
J Joukje JJ Siebenga,
Harry H Vennema,
Du-Ping DP Zheng,
Jan J Vinjé,
Bonita E BE Lee,
Xiao-Li XL Pang,
Eric C M EC Ho,
Wilina W Lim,
Avinash A Choudekar,
Shobha S Broor,
Tamar T Halperin,
Nassar B G NB Rasool,
Joanne J Hewitt,
Gail E GE Greening,
Miao M Jin,
Zhao-Jun ZJ Duan,
Yalda Y Lucero,
Miguel M O'Ryan,
Marina M Hoehne,
Eckart E Schreier,
Rodney M RM Ratcliff,
Peter A PA White,
Nobuhiro N Iritani,
Gábor G Reuter and
Marion M Koopmans
Abstract
NoV epidemics resulted from the global spread of GII.4 strains that evolved under the influence of population immunity. Lineages show notable (and currently unexplained) differences in geographic prevalence. Establishing a global NoV network by which data on strains with the potential to cause pande...
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PMID: 19627248
PDF is available here.
Abstract
We conducted a molecular biological survey of NoVs existing in sewage water in Toyama Prefecture, Japan. Although GII/4 was predominantly detected in sewage water that was associated with a high frequency of outbreaks caused by this genotype, other genotypes, including two types of recombinant strai...
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PMID: 19762994
PDF is available here.
Abstract
Proanthocyanidin, which consists of (+) catechin, (-) epicatechin and their gallates (15%), (-) epicatechin gallate-dimers, -trimers, and -tetramers (80%), and (-) epicatechin gallate-pentamers, -hexamers, and -heptamers (5%), was evaluated for its antiviral activity against feline calicivirus F9 st...
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PMID: 19785283
PDF is available here.
Abstract
Recently, environmental swabs from kitchen and bathroom surfaces have been described as an additional tool for the detection of norovirus in outbreak settings. This article describes an outbreak investigation in response to the reporting of gastroenteritis in three unrelated groups of 6, 12, and 13...
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PMID: 19722414
PDF is available here.
Abstract
Norovirus was a frequent cause of acute severe sporadic gastroenteritis in children representing the second etiologic agent after rotavirus....
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PMID: 19561424
PDF is available here.
Abstract
We determined the role of pH during MNV-1 infection since the low pH environment of endosomes often triggers uncoating of viruses. We demonstrated that MNV-1 viral titers by plaque assay and expression of the non-structural protein VPg by immunofluorescence were not affected by pH in cultured and pr...
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PMID: 19463729
PDF is available here.
Abstract
The efficiency of sodium hypochlorite (NaOCl) and peroxyacetic acid (PAA) to reduce murine norovirus 1 (MNV-1), a surrogate for human norovirus, and Bacteroides fragilis HSP40-infecting phage B40-8 on shredded iceberg lettuce was investigated. The levels of removal of viruses MNV-1 and B40-8 were co...
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PMID: 19517733
PDF is available here.
Abstract
Acute gastroenteritis caused by viruses is one of the leading causes of infantile morbidity. The aim of the present study was to investigate the presence of human caliciviruses of the genera norovirus and sapovirus in children up to 3 years of age with acute gastroenteritis from low-income communiti...
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PMID: 19377793
PDF is available here.
Abstract
Stool specimens were provided by 306 patients (161 female) with acute diarrhea. The ages of the patients ranged from 1 to 89 years (mean 37, median 36). Pathogens were detected in 71 (23.2%) patients, with incidence peaks in February and June. Norovirus accounted for 36.0% of positive results, C. di...
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PMID: 19148576
PDF is available here.