Abstract
We measured the effects of diet on protein concentration, hypopharyngeal gland development and virus titers in worker honey bees fed either pollen, a protein supplement (MegaBee), or a protein-free diet of sugar syrup. Workers consumed more pollen than protein supplement, but protein amounts and siz...
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PMID: 20346950
PDF is available here.
Abstract
We have evaluated the antitumor activity of NTX-010 administered systemically.
In vitro NTX-010 was tested against 23 cell lines exposed for 96 hr at 1 x 10(-4) to 10(4) viral particles (vp)/cell. In vivo NTX-010 was administered intravenously once at 3 x 10(12) vp/kg. Three measures of antitumor ac...
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PMID: 20582972
PDF is available here.
Abstract
We found that the 5' termini of the transcripts are diphosphorylated. The 5'-terminal nucleotide is G, and GDP was the best substrate among those examined to prime the reaction. When GTP was used, the triphosphate of GTP incorporated into the 5'-end was converted to diphosphate. This activity was no...
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PMID: 20511225
PDF is available here.
Abstract
We show that Bunyamwera virus (BUNV), the prototype of the Bunyaviridae family of segmented negative-sense RNA viruses, also can repair its genomic termini. When an intact nontranslated region (NTR) was added to the anti-genomic 3' end, it was precisely removed, to restore both length and RNA synthe...
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PMID: 20430858
PDF is available here.
Abstract
Respiratory viruses contribute to the seasonal pattern of invasive pneumococcal disease (IPD), but the impact of viral coinfections on the clinical characteristics and outcomes of patients with IPD have not been well defined.
This study was designed to describe and compare the clinical presentations...
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PMID: 20051928
PDF is available here.
Abstract
Live-cell fluorescence microscopy was used to investigate the third triple gene block protein (TGB3) of potato mop-top pomovirus and its role in assisted targeting of TGB2 to plasmodesmata (PD). Wild-type and mutant TGB3 proteins were expressed under the control of the 35S promoter or from a virus r...
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PMID: 20350737
PDF is available here.
Abstract
A retrospective serosurvey of multi-host feline and canine viruses among carnivore species in southern Africa (n=1018) identified widespread pathogen exposure even in remote protected areas. In contrast to mortality experienced in East African predators, canine distemper virus (CDV) infection among...
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PMID: 19038454
PDF is available here.
Abstract
Quantitative multiplex real-time RT-PCR assays utilizing fluorescence resonance energy transfer (FRET) hybridization probes were developed for the detection of 13 respiratory viruses, including well recognized viral causes (respiratory syncytial virus, influenza viruses A and B, parainfluenza viruse...
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PMID: 20153377
PDF is available here.
Michele Tonelli,
Matteo Simone,
Bruno Tasso,
Federica Novelli,
Vito Boido,
Fabio Sparatore,
Giuseppe Paglietti,
Sabrina Pricl,
Gabriele Giliberti,
Sylvain Blois,
Cristina Ibba,
Giuseppina Sanna,
Roberta Loddo and
Paolo La Colla
Abstract
Seventy-six 2-phenylbenzimidazole derivatives were synthesized and evaluated in cell-based assays for cytotoxicity and antiviral activity against a panel of 10 RNA and DNA viruses. The most commonly affected viruses were, in decreasing order, CVB-2, BVDV, Sb-1, HSV-1, and YFV, while HIV-1 and VSV we...
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PMID: 20359898
PDF is available here.
Abstract
Experimental evidence that RNA virus populations consist of distributions of mutant genomes, termed quasispecies, was first published 31 years ago. This work provided the earliest experimental support for a theory to explain a system that replicated with limited fidelity and to understand the self-o...
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PMID: 20152841
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
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PMID: 20033838
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
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PMID: 20033838
PDF is available here.
Abstract
These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did not have the npt II gene. DNA sequences flanking T-DNA insertions in the T(2) generation were determined usi...
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PMID: 19557540
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
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PMID: 20033838
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
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PMID: 20033838
PDF is available here.
Abstract
These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did not have the npt II gene. DNA sequences flanking T-DNA insertions in the T(2) generation were determined usi...
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PMID: 19557540
PDF is available here.
Abstract
These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did not have the npt II gene. DNA sequences flanking T-DNA insertions in the T(2) generation were determined usi...
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PMID: 19557540
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
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PMID: 20033838
PDF is available here.
Abstract
These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did not have the npt II gene. DNA sequences flanking T-DNA insertions in the T(2) generation were determined usi...
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PMID: 19557540
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
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PMID: 20033838
PDF is available here.
Abstract
These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did not have the npt II gene. DNA sequences flanking T-DNA insertions in the T(2) generation were determined usi...
|
PMID: 19557540
PDF is available here.
Abstract
These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did not have the npt II gene. DNA sequences flanking T-DNA insertions in the T(2) generation were determined usi...
|
PMID: 19557540
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
|
PMID: 20033838
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
|
PMID: 20033838
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
|
PMID: 20033838
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
|
PMID: 20033838
PDF is available here.
Abstract
These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did not have the npt II gene. DNA sequences flanking T-DNA insertions in the T(2) generation were determined usi...
|
PMID: 19557540
PDF is available here.
Abstract
Solenopsis invicta virus 3 (SINV-3) is a recently described positive-strand RNA virus that infects the red imported fire ant, S. invicta. The genome of an Argentinean isolate of Solenopsis invicta virus 3 (SINV-3(ArgSF )) obtained from the Santa Fe region of Argentina was sequenced i...
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PMID: 20033838
PDF is available here.
Abstract
We measured the prevalence of four barley and cereal yellow dwarf viruses (B/CYDVs) in three grass species at 26 natural grasslands along a 2000-km latitudinal gradient in the western United States and Canada. B/CYDVs are aphid-vectored RNA viruses that cause one of the most prevalent of all plant d...
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PMID: 20426331
PDF is available here.
Abstract
We report the complete Euprosterna elaeasa virus (EeV) genome sequence of 5698 nt with no poly(A) tail and two overlapping open reading frames, encoding the replicase and capsid precursor, with approximately 67% amino acid identity to Thosea asigna virus (TaV). The N-terminally positioned 17 kDa pro...
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PMID: 19954807
PDF is available here.
Abstract
This study evaluated the susceptibility of Penaeus vannamei, Kona stock-line, to infection by an isolate of Taura syndrome virus from Belize (TSV-BZ) under hyperthermic conditions (32 degrees C). Shrimp exposed to the reference Hawaii-94 isolate of TSV (TSV-HI) showed resistance to infection at 32 d...
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PMID: 20225676
PDF is available here.
Abstract
We perform Brownian dynamics on a coarse-grained model that describes the dynamics of icosahedral capsid assembly around a flexible polymer. We identify several mechanisms by which the polymer plays an active role in its encapsulation, including cooperative polymer-protein motions. These mechanisms...
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PMID: 21149971
PDF is available here.
Abstract
Being infected with RSV was associated with a more severe clinical presentation of pneumonia, longer illness duration, and increased risk of treatment failure. The severity of RSV infection was age related, infants being more severely affected....
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PMID: 19935451
PDF is available here.
Abstract
To construct eukaryotic expression vector of general type in reverse genetics systems of non-segmented negative strand RNA viruses, the multiple cloning site of the plasmid pVAX1 was replaced with HamRz cDNA sequence, a 9 sites linker and HdvRz cDNA sequence through the sequential addition of three...
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PMID: 20329561
PDF is available here.
Abstract
We show that Sua5 is critical for normal translation. The loss of SUA5 causes increased leaky scanning through AUG codons, +1 frameshifting, and nonsense suppression. In addition, the loss of SUA5 amplifies the 20S RNA virus found in Saccharomyces cerevisiae, possibly through an internal ribosome en...
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PMID: 19884342
PDF is available here.
Abstract
We have previously shown that the heat shock protein 40 (hsp40) chaperone encoded by the yeast YDJ1 gene facilitates RNA replication of flock house virus (FHV), a well-studied and versatile positive-sense RNA model virus. To further explore the roles of chaperones in FHV replication, we examined a p...
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PMID: 19828623
PDF is available here.
Abstract
We measured the metabolic rate and respiration of C. parasitica colonies grown on solid medium. These experiments supported historical observations of other fungal species done in liquid cultures that the metabolic rate steadily declines with age and differentiation of the mycelium. Hypovirus infect...
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PMID: 19589830
PDF is available here.
Abstract
Many studies suggest strongly that viral infections may predispose patients to the development of asthma and other atopic diseases. Further, mechanistic studies are necessary to allow for the development of targeted therapeutics to prevent the translation of viral into atopic disease....
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PMID: 19788013
PDF is available here.
Abstract
I is recognized as G instead of A, A-to-I editing by ADAR1 can lead to genetic recoding and altered RNA structures. The importance of PKR and ADAR1 in innate antiviral immunity is illustrated by a number of viruses that encode either RNA or protein viral gene products that antagonize PKR and ADAR1 e...
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PMID: 19715457
PDF is available here.
Abstract
We also address the types of pneumonia caused by hantaviruses, metapneumoviruses and rhinoviruses....
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PMID: 19820817
PDF is available here.
Abstract
I use case studies of 3 RNA viruses to explore the impact of genomic sequence data, with particular emphasis on influenza A virus. Notably, the studies of RNA virus genomics undertaken to date largely focused on issues of evolution and epidemiology, and they have given these disciplines new impetus....
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PMID: 19729846
PDF is available here.
Abstract
Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the RÃa de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovir...
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PMID: 19784920
PDF is available here.
Abstract
We report an outbreak of viral gastroenteritis linked to municipal drinking water in a town in northern Italy in June 2009. Over one month we identified 299 probable cases of whom 30 were confirmed for at least one of the following viruses: norovirus, rotavirus, enterovirus or astrovirus. Water samp...
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PMID: 19643050
PDF is available here.
Michele Tonelli,
Iana Vazzana,
Bruno Tasso,
Vito Boido,
Fabio Sparatore,
Maurizio Fermeglia,
Maria Silvia Paneni,
Paola Posocco,
Sabrina Pricl,
Paolo La Colla,
Cristina Ibba,
Barbara Secci,
Gabriella Collu and
Roberta Loddo
Abstract
Twelve aminoarylazocompounds (A-C) and 46 aryltriazene 7 derivatives (D-G) have been synthesized and evaluated in cell-based assays for cytotoxicity and antiviral activity against a panel of 10 RNA and DNA viruses. Eight aminoazocompounds and 27 aryltriazene derivatives exhibited ant...
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PMID: 19482481
PDF is available here.
Abstract
These results indicate that the organic-based method is more suitable for detecting viruses in sewage sludge....
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PMID: 19722079
PDF is available here.
Abstract
I was the most prevalent (56%), followed by group IV (25%), group III (10%) and group II (9%). Group III coliphages were only detected at the sampling site in the vicinity of the international boundary, indicating human faecal contamination. As the New River traverses through the US region, groups I...
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PMID: 19240353
PDF is available here.
Abstract
At present, more than 40 protist-infecting viruses have been isolated and characterized. From the viewpoints of molecular ecology, taxomony and molecular evolution, several new discoveries were made within the last five years. In this minireview, three topics of interest on protist-infecting viruses...
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PMID: 19927986
PDF is available here.
Abstract
Asthma prevalence has markedly increased over the past 30 years. Although atopy and exposure to environmental allergens are known to exacerbate asthma, recent literature supports a causal role of indoor allergens in disease development.
High-risk birth cohorts continue to point to atopy as the main...
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PMID: 19326507
PDF is available here.
Abstract
Our data support the usefulness of mPCR as a rapid molecular method for the simultaneous detection of major viral pathogens and T. gondii in aseptic meningitis also to allow the earlier application of specific antiviral therapy....
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PMID: 19579690
PDF is available here.
Abstract
We showed that the protein produced on translational readthrough (RT) of the coat protein (CP) gene, CP-RT, is associated with one extremity of the virus particles, and that the two RNAs encoding replicase and movement proteins can move long distance in the absence of the third RNA (RNA-CP) that enc...
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PMID: 19271953
PDF is available here.
Abstract
Fungal zoospores of Olpidium species transmit several viruses in the family Tombusviridae as well as in the Ophio- and Varicosavirus genera. This unit describes procedures for virus transmission by Olpidium sp. The method is useful for assessing fungal transmissibility of a given virus as well as fo...
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PMID: 19235147
PDF is available here.
Abstract
Virus diseases often are spread between plants by vector organisms, some of which live below ground (e.g., fungi and nematodes) and feed on the plant root system. Tobraviruses are one of only two groups of plant viruses that have nematode vectors. They are primarily viruses of weed plants but can ca...
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PMID: 19235148
PDF is available here.
Abstract
Programmed ribosomal frameshifting (PRF) is one of the multiple translational recoding processes that fundamentally alters triplet decoding of the messenger RNA by the elongating ribosome. The ability of the ribosome to change translational reading frames in the -1 direction (-1 PRF) is employed by...
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PMID: 18621088
PDF is available here.
Abstract
We describe the characteristics of dicistroviruses, including the RNA elements and viral proteins....
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PMID: 18621089
PDF is available here.
Abstract
Transfer RNA-like structures (TLSs) that are sophisticated functional mimics of tRNAs are found at the 3'-termini of the genomes of a number of plant positive strand RNA viruses. Three natural aminoacylation identities are represented: valine, histidine, and tyrosine. Paralleling this variety in str...
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PMID: 18638511
PDF is available here.