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Viruses, Unclassified (3):
Recent article
Viruses, Unclassified
Abstract
To develop a molecular biology method which can be applied to detect unknown virus fast and accurately.
The samples were separated from human cerebrospinal fluid by infected human rhabdomyosarcoma cells. After centrifugation and discarding the cell debris, the samples were subjected to ultracentrifu...
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PMID: 20077735
PDF is available here.
PDF is available here.
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Abstract
We describe a series of laboratory experiments aimed at assessing the likely modes of disease transmission, determining the survival of lobsters relative to each transmission pathway and identifying potential alternate hosts. Given evidence for lower prevalence of PaV1 in large lobsters, the effect...
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PMID: 18589993
PDF is available here.
PDF is available here.
Virus Research (132)1-2 2008
Abstract
The complete sequences of the two major RNAs of Chronic bee paralysis virus (CBPV) have been determined. RNA 1 (3674nt long) and RNA 2 (2305nt long) are positive single-stranded RNAs that are capped but not polyadenylated. The 3' ends of both RNAs are unreactive to polymerisation or ligation even in...
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PMID: 18079012
PDF is available here.
PDF is available here.
Virus Research (132)1-2 2008
Abstract
We examined the histopathological and hematological response of the Caribbean spiny lobster to experimentally induced infections with Panulirus argus Virus 1 (PaV1). The fixed phagocytes in the hepatopancreas were the primary sites of PaV1 infection in spiny lobsters. Fixed phagocytes were activated...
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PMID: 18177971
PDF is available here.
PDF is available here.
Abstract
We suggest that Antarctic lakes may be a reservoir of previously undescribed viruses that possess novel biological and biochemical characteristics....
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PMID: 11543124
PDF is available here.
PDF is available here.
Abstract
This paper deals with some aspects of human viruses in the environment. Thus, a systematization of these viruses was made on the basis of their elimination from the human body and also with regard to their detection in various segments of the environment. The data compiled lead to the conclusion tha...
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PMID: 7655410
PDF is available here.
PDF is available here.
Veterinary Microbiology (42)4 1994
Abstract
We investigated the growth of Lelystad virus (LV) in porcine alveolar macrophages, the thermal and pH stability of the virus in cell culture medium, and its survival in tissue specimens from viraemic pigs. Lelystad virus grew to titres of 10(6) TCID50/ml, which were found at 40 h after virus inocula...
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PMID: 9133061
PDF is available here.
PDF is available here.
Journal of General Virology (75 ( Pt 7)) 1994
Abstract
Our results indicate that VR 2385, like LV, is a member of the newly proposed arterivirus group. However, the striking genetic variation and the difference in pathogenicity between LV and VR 2385 suggest that the viruses causing PRRS in the U.S.A. and Europe are highly variable and they may represen...
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PMID: 8021610
PDF is available here.
PDF is available here.
Folia Biologica (Prague) (39)4 1993
Abstract
Abstract
We showed that it is a two-component system. One part of the complex, MX, is exogenous; it is manifested by a protein, p58X, which is a cytoplasmic antigen and it reacts with some natural sera of man and of various animals. The other component, MN, is endogenous to human cells. This is manifested by...
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PMID: 1312272
PDF is available here.
PDF is available here.
Abstract
These results suggested that some small round viruses resembling astroviruses might show cytopathic effect in BS-C-1 cells and may be associated with an oyster-related gastroenteritis....
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PMID: 1658159
PDF is available here.
PDF is available here.
Journal of Infection (23)2 1991
Abstract
Veterinary Quarterly (13)3 1991
Abstract
We focused on isolating viruses and mycoplasmas, and we tested paired sera of affected sows for antibodies against ten known pig viruses. The mycoplasmas M. hyosynoviae, M. hyopneumoniae, and Acholeplasma laidlawii, and the viruses encephalomyocarditis virus and porcine enterovirus types 2 and 7 wer...
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PMID: 1835211
PDF is available here.
PDF is available here.
Veterinary Quarterly (13)3 1991
Abstract
We conclude that Lelystad virus is the causal agent of mystery swine disease. Since its aetiology is no longer a mystery, we propose the more appropriate name 'porcine epidemic abortion and respiratory syndrome (PEARS)'....
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PMID: 1949539
PDF is available here.
PDF is available here.
Abstract
The morphogenesis of viral haemorrhagic disease virus (VHDV) of rabbits in infected cell cultures and in tissues and cells from infected animals has been observed systematically by electron microscopy. Viral particles have been found to assemble through a condensation process within the nucleus of i...
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PMID: 1760588
PDF is available here.
PDF is available here.
Abstract
The first outbreaks of viral haemorrhagic disease (VHD) of rabbits were reported from eastern Slovakia in 1987. In 1988, the infection spread throughout the Czech and Slovak Federal Republic. Electron microscopy was used by the Veterinary Research Institute in Brno to diagnose the disease during the...
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PMID: 1760589
PDF is available here.
PDF is available here.
Abstract
We examined some epidemiological features of the viruses associated with gastrointestinal illness, using national data reported by electron microscopists in the United Kingdom. During the 3 years analyzed (1985-1987), a total of 1,993 positive detections of astroviruses, caliciviruses, coronaviruses...
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PMID: 1652619
PDF is available here.
PDF is available here.
Abstract
Methods aimed at the detection of causative agents in the CSF and peripheral blood lymphocytes are recommended for the use in intravital laboratory diagnosis of slow infections of the central nervous system. The results obtained enable recommending the biotest on guinea-pigs or indication of the cau...
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PMID: 1647639
PDF is available here.
PDF is available here.
Avian Diseases (35)3 1991
Abstract
Twelve-day-old broiler-type chickens had hemorrhagic necrotic wing tips. After 10 blind subcultures in an MDCC-MSB1 cell line, a virus (so-called chick anemia agent [CAA]) was isolated and designated CL-1 CAA. Five-day-old specific-pathogen-free chicken embryos from a commercial breeder flock that w...
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PMID: 1835375
PDF is available here.
PDF is available here.
Abstract
We reviewed 6 years of retrospective data on viral agents of gastroenteritis screened by electron microscopy at 10 centers in the United States and Canada. From 52,691 individual electron microscopic observations, a virus was detected in 16% of specimens, and the yearly positive detection rate among...
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PMID: 2172903
PDF is available here.
PDF is available here.
Abstract
Borna disease virus represents an unknown neurotropic agent. It causes encephalitis in horses and sheep. The same or a similar type of virus might be responsible for psychiatric disorders in man. So far, it has been impossible to purify this agent to such an extent that it could be analyzed biochemi...
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PMID: 2117915
PDF is available here.
PDF is available here.
Abstract
We report here the isolation and preliminary characterization of cDNA clones from a rat with BD. These clones hybridized to specific transcripts in BD rat brain and arrested in vitro translation of BD proteins. In situ hybridization experiments using RNA probes prepared from these clones showed an a...
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PMID: 1693432
PDF is available here.
PDF is available here.
Antiviral Research (13)4 1990
Abstract
We investigated the activity of halogeno-, cyano- and amidino-isoflavenes, isoflavans and flavans on the multiplication of human astroviruses. These are naked small round viruses which have been recognized as causative agents of human gastroenteritis, and whose capsid proteins are similar to those o...
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PMID: 2116755
PDF is available here.
PDF is available here.
Abstract
A variety of small round-structured viruses are being recognized with increasing frequency as a cause of gastroenteritis in the community, but have rarely been reported to cause outbreaks in hospitals or extended-care facilities. From March 20 through April 15, 1988, an outbreak of gastroenteritis o...
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PMID: 2107737
PDF is available here.
PDF is available here.
Abstract
A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cy...
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PMID: 2107200
PDF is available here.
PDF is available here.
Abstract
An enzyme-linked immunosorbent assay (ELISA), based on monoclonal antibodies to the astrovirus group antigen, was designed for the detection of astroviruses in stools of patients with gastroenteritis. Compared to immune electron microscopy used as the standard test, the sensitivity of the astrovirus...
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PMID: 2105359
PDF is available here.
PDF is available here.
Archives of Virology (113)1-2 1990
Abstract
We report conditions for the growth of human faecal astrovirus in a continuous colonic carcinoma cell line (CaCo-2). Purified particles contained three polypeptides, one of which (24k) appeared loosely held on the exterior.
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PMID: 2117433
PDF is available here.
PDF is available here.
Archives of Virology (110)3-4 1990
Abstract
We conclude that MCV can be serially propagated by techniques used for previously described astroviruses and is serotypically an astrovirus type 5....
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PMID: 2107802
PDF is available here.
PDF is available here.
Abstract
Journal of General Virology (70 ( Pt 12)) 1989
Abstract
Borna disease virus (BDV) is an unclassified agent that causes neurological disease in a wide range of animal species and possibly in humans. The infectious nature of BDV has been long established but, despite extensive progress on the pathogenesis of the infection, the aetiological agent is still u...
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PMID: 2514256
PDF is available here.
PDF is available here.
Biological Psychiatry (26)8 1989
Abstract
We monitored the neuronal functions of intracerebrally infected but healthy rats with three types of learning experiments. Spatial discrimination learning, using the y maze and the hole board, was significantly less successful in BD virus-infected (I) compared with mock-infected (M) rats. Similarly,...
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PMID: 2511930
PDF is available here.
PDF is available here.
Veterinary Record (125)21 1989
Abstract
The adequacy of a histopathological diagnosis of bovine spongiform encephalopathy (BSE) based exclusively on observations of neuroparenchymal vacuolation in three specific neuroanatomic nuclei was tested by using a standard coronal section of medulla oblongata cut at the obex. The agreement between...
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PMID: 2512718
PDF is available here.
PDF is available here.
Veterinary Record (125)21 1989
Abstract
Abstract
I micrometer-thick cryosections of hippocampus and sciatic nerve preparations revealed several cells that expressed both glial and BDV antigens. Infectious virus was recovered from cultures of Schwann cells from infected rats. Borna disease virus-infected glial elements persisted beyond the period o...
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PMID: 2507750
PDF is available here.
PDF is available here.
The Lancet (2)8669 1989
Abstract
A 32-nm small round structured virus (SRSV), possibly related to the Snow Mountain agent (SMA), was implicated as the cause of recurrent outbreaks of gastroenteritis on a cruise ship. There was no identifiable relation to food or water consumption, but the risk of gastroenteritis among passengers wh...
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PMID: 2571872
PDF is available here.
PDF is available here.
Psychiatry Research (29)3 1989
Abstract
Abstract
Small, round-structured virus (SRSV) was detected in a stool specimen of a patient during an acute gastroenteritis outbreak in Tokyo and was tentatively named SRSV-9. SRSV-9 was purified by sucrose velocity gradient centrifugation after CsCl density gradient centrifugation. The buoyant density of SR...
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PMID: 2504773
PDF is available here.
PDF is available here.
Journal of Virology (63)3 1989
Abstract
We present the first physical characterization of the Creutzfeld-Jakob disease agent. Preparations with high yields of infectivity (assayed infectious units) were obtained by a novel, gentle procedure in which initially sedimenting Gp34 ("prion" protein) was disaggregated by a variety of criteria wi...
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PMID: 2492609
PDF is available here.
PDF is available here.
Veterinary Microbiology (19)2 1989
Abstract
Bovine astrovirus serotype 2 (US2) was adapted to primary neonatal kidney cell (NBK) cultures by the addition of 50 micrograms ml-1 of trypsin in the medium. Infectious virus was released from the cells within 7 days post-infection in early passages and within 3 days in later passages. In the absenc...
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PMID: 2495698
PDF is available here.
PDF is available here.
Archives of Virology (108)1-2 1989
Abstract
We have been able to cultivate, 3 (types 1, 2, and 5) formed well-defined plaques in LLCMK2 cell cultures under an agar overlay containing trypsin. A virus neutralization assay based on plaque reduction was applied to these 3 serotypes. It was found that rabbit antisera prepared against individual s...
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PMID: 2512894
PDF is available here.
PDF is available here.
The Lancet (2)8612 1988
Abstract
Abstract
We readily distinguished between serotypes of astrovirus; however, these serotypes showed a high degree of cross-reactivity by enzyme-linked immunoassay, a result indicating the presence of a group antigen. We prepared monoclonal antibodies to astrovirus type 2 antigen and selected them on the basis...
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PMID: 3134491
PDF is available here.
PDF is available here.
Abstract
The development and evaluation of radioimmune assays for the detection of IgM and IgG antibodies to astrovirus are described. The test was shown to be sensitive and specific, and suitable for screening large numbers of sera. The use of the assays has established that astrovirus type 1 is prevalent i...
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PMID: 3130387
PDF is available here.
PDF is available here.
Journal of Virology (61)11 1987
Abstract
Borna disease virus is an uncharacterized agent that causes sporadic but fatal neurological disease in horses and sheep in Europe. Studies of the infection in rats have shown that the agent has a strict tropism for neural tissues, in which it persists indefinitely. Inoculated rats developed encephal...
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PMID: 2444715
PDF is available here.
PDF is available here.
Der Nervenarzt (58)10 1987
Abstract
Journal of Virology (61)10 1987
Abstract
Information was sought on the temporal distribution of transmissible mink encephalopathy virus in royal pastel mink inoculated subcutaneously with 10(3.0) 50% intracerebral lethal doses of the Idaho strain. As determined by intracerebral assay in mink, extremely little replication of the virus occur...
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PMID: 2957510
PDF is available here.
PDF is available here.
The Lancet (2)8561 1987
Abstract
Abstract
Annales de Pediatrie (34)7 1987
Abstract
Veterinary Record (120)23 1987
Abstract
Abstract
In a study to learn more about the pathogenicity of transmissible mink encephalopathy virus for the natural hosts of scrapie, 20 Cheviot sheep and 19 dairy goats were inoculated intracerebrally with the Idaho strain of the virus. Five sheep and nine goats became affected with a progressive neurologi...
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PMID: 2952237
PDF is available here.
PDF is available here.
Abstract
The name astrovirus was used by Madeley and Cosgrove in 1975 to describe a small round virus (approximately 28 nm diameter) with star-like appearance on electron microscopy. It was first seen in faeces from a few children with gastroenteritis. An aetiological role in gastroenteritis has since been c...
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PMID: 3109854
PDF is available here.
PDF is available here.
Abstract
The serotypes 1 and 2 of Breda virus from feces of experimentally infected gnotobiotic calves were studied with respect to their sedimentation and density properties in sucrose gradients and their structural polypeptides; Berne virus, the proposed prototype of the new family Toroviridae, was include...
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PMID: 3094412
PDF is available here.
PDF is available here.
Abstract
Enzyme-linked immunosorbent assays (ELISAs) for antigen detection and blocking ELISAs for serum antibody rises were developed for the Snow Mountain and Norwalk agents of viral gastroenteritis. The ELISAs were as sensitive as the existing radioimmunoassays and were specific for the Snow Mountain or N...
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PMID: 3020085
PDF is available here.
PDF is available here.
Abstract
Pleomorphic virus-like particles have been observed by electron microscopy in the faeces of children and adults with diarrhoea. Some of these particles were approximately 100 nm in diameter and had a "fringe" of closely applied peplomers approximately 10 nm long; they closely resembled Berne virus o...
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PMID: 3093635
PDF is available here.
PDF is available here.
Journal of General Virology (67 ( Pt 6)) 1986
Abstract
Nine isolates of pleural effusion disease agent or virus (PEDV) from treponema-infected rabbits in various countries were examined for pathogenicity and persistence in rabbits. The isolates showed a wide range of pathogenicity and were categorized into three groups according to the severity of the a...
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PMID: 3086501
PDF is available here.
PDF is available here.
Abstract
Journal of Virology (58)2 1986
Abstract
We purified and characterized SMA directly from human stool specimens containing the virus. The density of the SMA virion was 1.29 g/cm3 and 1.21 to 1.22 g/cm3 on potassium tartrate-glycerol gradients and 1.33 to 1.34 g/cm3 on cesium chloride gradients. SMA had an S value of 170 to 183S on a sucrose...
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PMID: 3009862
PDF is available here.
PDF is available here.
Journal of General Virology (67 ( Pt 2)) 1986
Abstract
A virus-specific antigen was extracted from brains of rats and from MDCK cells infected with Borna disease (BD) virus and purified to homogeneity by immunoaffinity chromatography and HPLC. The antigen consists of two components which are almost equal in size (38 000 mol. wt.), and it forms aggregate...
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PMID: 3080548
PDF is available here.
PDF is available here.
Abstract
TME from a single source was transmitted by intracerebral injection to Chinese hamsters, producing clinical disease in all seven animals after incubation periods of over 600 days. The brain from each of the primary cases was used to establish separate intracerebral passage-lines of TME and this led...
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PMID: 2940469
PDF is available here.
PDF is available here.