Advanced search×

Characterization of a Model Compound for the Lysine Tyrosylquinone Cofactor of Lysyl Oxidase

M M Akagawa and K K Suyama

Biochem Biophys Res Commun 281(1):7 (2001) PMID 11178979

We characterized a model compound for the lysine tyrosylquinone (LTQ) cofactor of lysyl oxidase which is one of the mammalian copper-dependent amine oxidases. The model compound, 4-butylamino-5-methyl-o-quinone, was prepared from n-butylamine and 4-methylcatechol by the oxidation with sodium iodate and characterized by spectroscopic analyses. The absorption maximum at 494 nm is consistent with that of lysyl oxidase. The model compound was capable of deaminating benzylamine to benzaldehyde at 37^oC in buffered aqueous acetonitrile. The aldehyde production was markedly elevated in the presence of the Cu(II)-EDTA complex but inhibited by free Cu(II). The catalytic cycle was observed at pH 10 in the presence of Cu(II), and the pH activity profile showed a broad optimum at about pH 9.0. In the presence of @b-aminopropionitrile and upon deoxygenation with N"2 aldelyde, production was decreased. The important features of the reaction were consistent with the enzymatic reaction.

Copyright © 2001 Elsevier Ltd. All rights reserved.

DOI: 10.1006/bbrc.2001.4315
Version: za2963e q8zad q8zbb q8zc3 q8zd1 q8ze1 q8zfd q8zg0

Similar articles you may find interesting…

  1. Pressure-induced magnetization reversal in the lightly electron-doped manganite compound (Ca,Sr)Mn$_{0.95}$Sb$_{0.05}$O$_{3}$
    Takahiro Fujiwara, Michiaki Matsukawa, and Ramanathan Suryanarayanan

    arXiv:1305.4708 [cond-mat.str-el] 21 May 2013

    We report here on the $dc$ magnetization as a function of temperature and Pressure up to 1 GPa in the lightly electron-doped manganite compound (Ca$_{1-x}$Sr$_{x}$)Mn$_{0.95}$Sb$_{0.05}$O$_{3}$ with its fixed carrier Content. When the magnetic field is small ($<$500 Oe), diamagnetic Magnetization is...
    View PDF
  2. Alpha-reliable combined mean traffic equilibrium model with stochastic travel times
    Wenyi Zhang, Wei Guan, and Huijun Sun

    arXiv:1305.4795 [math.OC] 21 May 2013

    Based on the reliability budget and percentile travel time (PTT) concept, a new travel time index named combined mean travel time (CMTT) under stochastic traffic network was proposed. CMTT here was defined as the convex combination of the conditional expectations of PTT-below and PTT-excess travel...
    View PDF
  3. Characterization of hereditarily reversible posets
    Micha{\l} Kukie{\l}a

    arXiv:1305.5085 [math.CO] 22 May 2013

    We give a complete characterization of Hereditarily reversible posets in terms of forbidden subsets. A similar result Is stated also for preordered sets. As a corollary we extend the list of known Examples of hereditarily reversible topological spaces....
    View PDF