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Characterization of a Model Compound for the Lysine Tyrosylquinone Cofactor of Lysyl Oxidase

M M Akagawa and K K Suyama

Biochem Biophys Res Commun 281(1):7 (2001) PMID 11178979

We characterized a model compound for the lysine tyrosylquinone (LTQ) cofactor of lysyl oxidase which is one of the mammalian copper-dependent amine oxidases. The model compound, 4-butylamino-5-methyl-o-quinone, was prepared from n-butylamine and 4-methylcatechol by the oxidation with sodium iodate and characterized by spectroscopic analyses. The absorption maximum at 494 nm is consistent with that of lysyl oxidase. The model compound was capable of deaminating benzylamine to benzaldehyde at 37^oC in buffered aqueous acetonitrile. The aldehyde production was markedly elevated in the presence of the Cu(II)-EDTA complex but inhibited by free Cu(II). The catalytic cycle was observed at pH 10 in the presence of Cu(II), and the pH activity profile showed a broad optimum at about pH 9.0. In the presence of @b-aminopropionitrile and upon deoxygenation with N"2 aldelyde, production was decreased. The important features of the reaction were consistent with the enzymatic reaction.

Copyright © 2001 Elsevier Ltd. All rights reserved.

DOI: 10.1006/bbrc.2001.4315
Version: za2963e q8zad q8zbb q8zc3 q8zd1 q8ze1 q8zfd q8zg0

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