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Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification.

A A Hiraishi

Lett Appl Microbiol 15(5):210-3 (1992) PMID 1280147

The 16S rRNA gene from various bacterial cultures was amplified by the polymerase chain reaction without DNA purification, and sequenced directly by using a laser fluorescent DNA sequencer and Tth polymerase with a cycle sequencing protocol. The described procedures provide almost complete 16S rDNA sequence data within a couple of days and facilitate systematic studies.

Referenced by 1 articles

DOI: 10.1111/j.1472-765X.1992.tb00765.x
Version: za2963e q8za8 q8zba q8zcd q8zd9 q8zea q8zf5 q8zg0

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