As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to the FLJ collection. About half of them (5,416) seemed to be protein-coding. Of those, 1,999 clusters had not been predicted by computational methods. The distribution of GC content of nonpredicted cDNAs had a peak at approximately 58% compared with a peak at approximately 42%for predicted cDNAs. Thus, there seems to be a slight bias against GC-rich transcripts in current gene prediction procedures. The rest of the cDNAs unique to the FLJ collection (5,481) contained no obvious open reading frames (ORFs) and thus are candidate noncoding RNAs. About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs.
We systematically overexpressed 99 transcription factor genes with the nmt1 promoter and found that 64 transcription factor genes exhibited reduced fitness when ectopically expressed. Cell cycle defects were also often observed. We further investigated three uncharacterized transcription factor gene...
A novel approach which exploits such correlation through the use of an
Eigensolver based on subspace iteration and accelerated with Chebyshev
Polynomials. The resulting solver, parallelized using the Elemental library
Framework, achieves excellent scalability and is competitive with curre...
We explore an approach for studying epistasis
In humans using a Drosophila melanogaster model of neonatal diabetes mellitus.
Expression of mutant preproinsulin, hINSC96Y, in the eye imaginal disc mimics
The human disease activating conserved cell stress response pathways leading to
Cell death and re...
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