Effects of high platelet concentration in collecting and freezing dry platelets concentrates

Transfus Apher Sci 30(3):8 (2004) PMID 15172625

Objective. To evaluate, in vitro, the effects of collecting and cryopreserving fresh dry platelet concentrates (PCs).Material and methods. Standard and dry PCs were collected in the same apheresis procedure. PCs were evaluated by mean platelet volume (MPV), pH, glucose and LDH levels. Activation was examined by flow cytometry using anti-CD41, anti-CD42 and anti-CD62p monoclonal antibodies and annexin binding assay. Platelet function was assessed by aggregation using ADP, collagen and arachidonic acid as agonists. Dry PCs were compared to standard PCs and to cryopreserved dry PCs. We also compared the use of ThromboSol (TS) to 5% DMSO as cryoprotectives.Results. Dry PCs presented a significantly reduced pH and glucose (p<0.001) and a higher percentage of annexin binding (p=0.005), in addition to a lower number of CD42 positive platelets (p=0.01).Conclusion. Dry PCs should be rapidly frozen after collection to avoid a fall in pH and platelet activation. 5% DMSO performed better than TS to cryopreserve dry PCs.

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