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LC-MS determination of desmopressin acetate in human skin samples

Melkamu Getie and Reinhard H H Neubert

J Pharm Biomed Anal 35(4):7 (2004) PMID 15193737

A sensitive, selective and accurate high-performance liquid chromatography-mass spectroscopy (LC-MS) assay for the determination of desmopressin acetate (1-deamino-8-d-arginine vasopressin, DDAVP) from human skin samples was developed and validated. Pieces of human breast skin were impregnated with DDAVP solutions and DDAVP was extracted with an optimum extraction procedure. The extracted solutions were then analyzed by a LC system, comprising of a Nucleosil^(R) C18 column (CC 125/2, 120-3) and a mobile phase of 0.01% formic acid in a mixture of 1.6mM ammonium acetate and acetonitrile (33:67, v/v), coupled with electrospray ionization mass spectrometry (ESI-MS). Satisfactory results were obtained with limits of detection and quantification as low as 10 and 40ng/ml, respectively, and with very good intra- and inter-day repeatability.

Copyright © 2004 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.jpba.2004.02.009
Version: za2963e q8za3 q8zbc q8zc4 q8zd1 q8ze1 q8zf8 q8zg2

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