The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC"5"0 value relative to the control. Increase in ^3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P0 values obtained for the epithelium. The present study clearly demonstrates that for a correlation between toxicity and permeability enhancement, both studies should be performed on the epithelium.
We investigate the effect of context on usability evaluation.
The focus is on how children behave and perform when they are tested in
Different settings. Two most commonly applied usability evaluation methods: the
Think-aloud and constructive interactions are applied to the children in
We can estimate the form dimension through the
Boundary dimension and vice versa. Second, I derive the proper scales of
Fractal dimension: the form dimension comes between 1.5 and 2, and the boundary
Dimension comes between 1 and 1.5. Third, I derive three form dimension values
With special geometri...
We propose a method for tailoring the absorption in a photonic
Crystal membrane. For that purpose, we first applied Time Domain Coupled Mode
Theory to such a subwavelength membrane and demonstrated that 100% resonant
Absorption can be reached even for a symmetric membrane, if degenerate modes
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