We have found that distamycin A can bind to DNA duplexes containing the (6-4) photoproduct, one of the major UV lesions in DNA, despite the changes, caused by photoproduct formation, in both the chemical structure of the base moiety and the local tertiary structure of the helix. A 20-mer duplex containing the target site, AATT.AATT, was designed, and then one of the TT sequences was changed to the (6-4) photoproduct. Distamycin binding to the photoproduct-containing duplex was detected by CD spectroscopy, whereas specific binding did not occur when the TT site was changed to a cyclobutane pyrimidine dimer, another type of UV lesion. Distamycin binding was analyzed in detail using 14-mer duplexes. Curve fitting of the CD titration data and induced CD difference spectra revealed that the binding stoichiometry changed from 1:1 to 2:1 with photoproduct formation. Melting curves of the drug-DNA complexes also supported this stoichiometry.
We present new algorithms for the problem of multiple string matching of
Gapped patterns, where a gapped pattern is a sequence of strings such that
There is a gap of fixed length between each two consecutive strings. The
Problem has applications in the discovery of transcription factor binding sites...
Real time laser induced fluorescence spectroscopy usage for microanalysis of
DNA double helix defects is shown. The method is based on Forster resonance
energy transfer (FRET) in intercalator-donor pair (acridine orange as a donor
and ethidium bromide as an acceptor). Transition metal ions such as...
We can hypothesize that a high frequency of the AhRR SNP can be a risk factor in the incidence of oral cancer and other neoplasias in PNG due to higher inducibility of CYP1A2. The potential role of AhRR pharmacogenetics in the risk of developing cancers associated with betel quid chewing and smoking...
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