Advanced search×

Human H9 cells proliferation is differently controlled by Vasoactive Intestinal Peptide or Peptide Histidine Methionine: implication of a GTP-insensitive form of VPAC"1 receptor

Stephanie Goursaud, Nicolas Pineau, Laetitia Becq-Giraudon, Pierre Gressens, Jean-Marc Muller, and Thierry Janet

J Neuroimmunol 158(1-2):12 (2005) PMID 15589042

The proliferation of human lymphoblastoma cell line (H9) was differently stimulated by Peptide Histidine Methionine (PHM) and Vasoactive Intestinal Peptide (VIP). PHM induced a cyclic AMP (cAMP) accumulation, abolished by Adenylate Cyclase (AC) inhibitors leading to a loss of proliferative effect. VIP mitogenic activity was Pertussis toxin (PTX) sensitive and AC inhibitors insensitive. Pharmacological experiments performed on H9 membranes with or without a GTP analogue indicated expression of both GTP-insensitive and -sensitive PHM/VIP high-affinity binding sites (HA). H9 cells expressed only the VPAC"1 receptor. VIP(10-28), known as a VPAC"1 antagonist, bond to all GTP-insensitive PHM sites and inhibited evenly the PHM and VIP mitogenic actions. These data strongly suggested different mechanisms initiated by VIP and PHM and highlighted the key role of GTP-insensitive binding sites in the control of cell proliferation.

DOI: 10.1016/j.jneuroim.2004.08.018
Version: za2963e q8za0 q8zba q8zce q8zd2 q8ze0 q8zfd q8zga

Similar articles you may find interesting…

  1. The good, the bad and the ugly — TFH cells in human health and disease
    Stuart G Tangye, Cindy S Ma, and Elissa K Deenick

    Nat Rev Immunol 13(6):412-426 (2013)

  2. Celastrol exerts synergistic effects with PHA-665752 and inhibits tumor growth of c-Met-deficient hepatocellular carcinoma in vivo.
    Hong-Lei Jiang, Jun-Zhe Jin, and Jian Liang

    Mol Biol Rep (2013) PMID 23649759

    We investigated the potential anti-human c-Met-deficient hepatocellular carcinoma effects of Celastrol, a novel triterpene, and its combination with PHA. Human hepatocellular carcinoma cells BEL-7402 (c-Met-positive) and Huh7 (c-Met-deficient) were treated with different dose of PHA with or without...
  3. The metastatic microenvironment: Lung-derived factors control the viability of neuroblastoma lung metastasis.
    Shelly Maman, Liat Edry-Botzer, and Isaac P Witz

    Int J Cancer (2013) PMID 23649556

    We were able to generate two neuroblastoma cell populations - lung micrometastatic (MicroNB) cells and lung macrometastatic (MacroNB) cells. These two types of cells share the same genetic background, invade the same distant organ, but differ in their ability to create metastasis in the lungs. We hy...