Advanced search×

Validation and implementation of a liquid chromatography/tandem mass spectrometry assay to quantitate dimethyl benzoylphenylurea (BPU) and its five metabolites in human plasma and urine for clinical pharmacology studies

Michelle A Rudek, Ming Zhao, Ping He, Yelena Zabelina, Runyan Jin, Wells A Messersmith, Antonio C Wolff, and Sharyn D Baker

J Chromatogr B Analyt Technol Biomed Life Sci 828(1-2):14 (2005) PMID 16253576

A method has been developed for the quantitation of N-[4-(5-bromo-2-pyrimidinyloxy)-3-methylphenyl]-N'-(2-dimethylamino-benzoyl)urea (BPU) and its metabolites in human plasma and urine. BPU and metabolites were separated on a C18 column with acetonitrile-water mobile phase containing 0.1% formic acid using isocratic flow for 5min. The analytes were monitored by tandem mass spectrometry. Calibration curves were generated over the range of 2.5-500ng/mL for BPU, mmBPU, and aminoBPU in plasma; and 0.1-20, 0.1-20, 0.5-100, 10-2000, 1-200, and 3-600ng/mL for BPU, mmBPU, aminoBPU, G280, G308, and G322 in urine, respectively. The method has been successfully applied to study the pharmacokinetics of BPU.

DOI: 10.1016/j.jchromb.2005.09.027
Version: za2963e q8za3 q8zb0 q8zc3 q8zd2 q8zee q8zf7 q8zg4

Similar articles you may find interesting…

  1. Metabolite identification of a new tyrosine kinase inhibitor, HM781-36B, and a pharmacokinetic study by liquid chromatography/tandem mass sp...
    Eunyoung Kim, Hankyong Kim, and Jongki Hong

    Rapid Commun Mass Spectrom 27(11):1183-95 (2013) PMID 23650031

    We validated an analytical method for HM781-36B and its major metabolites in plasma. In vivo and in vitro metabolism of HM781-36B was studied in dog plasma, urine and feces as well as using human and dog liver microsomes with extraction by ethyl acetate or methyl tert-butyl ether, re...
  2. Large-Scale Analysis of Peptide Sequence Variants: The Case for High-Field Asymmetric Waveform Ion Mobility Spectrometry.
    Andrew J Creese, Jade Smart, and Helen J Cooper

    Anal Chem (2013) PMID 23646896

    We present a systematic study of the benefits of FAIMS for a key challenge in proteomics, that of peptide isomers. We have applied FAIMS to the analysis of a phosphopeptide library comprising the sequences GPSGXVpSXAQLX(K/R) and SXPFKXpSPLXFG(K/R), where X = ADEFGLSTVY. The library has defined limit...
  3. Identification of Sinensetin Metabolites in Rat Urine by Isotope-Labeling Method and Ultrahigh-Performance Liquid Chromatography-Electrospra...
    Guor-Jien Wei, Jenn-Feng Sheen, and Ching-I Lin

    J Agric Food Chem (2013) PMID 23647150

    Sinensetin (SIN), one of the major polymethoxyflavones (PMFs) contained mainly in the citrus peels, has been reported possessing various bioactivities including antifungal, antimutagenic, anticancer, and anti-inflammatory activities. Although the biotransformation of SIN in fungi and...