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Evaluation of denaturing high-performance liquid chromatography as a rapid detection method for identification of epidermal growth factor receptor mutations in nonsmall-cell lung cancer.

Victor V Cohen, Jason S JS Agulnik, Jonathan J Jarry, Gerald G Batist, David D Small, Harvey H Kreisman, Neely Adriana NA Tejada, Wilson H WH Miller, and George G Chong

Cancer 107(12):2858-65 (2006) PMID 17096434

Somatic mutations of the epidermal growth factor receptor (EGFR) gene in nonsmall-cell lung cancer (NSCLC) may predict responsiveness to tyrosine kinase inhibitors. These mutations are commonly identified using DNA sequencing methods. Although considered the gold standard, this approach is time-consuming. In addition, this approach requires large diagnostic specimens and a high ratio of tumor-to-normal-tissue DNA for optimal results. The use of denaturing high-performance liquid chromatography (dHPLC) as a method to screen for the 2 predominant EGFR mutations is reported.

DOI: 10.1002/cncr.22331
Version: za2963e q8zac q8zb7 q8zcf q8zd2 q8ze2 q8zf5 q8zg6

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