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Preparative isolation and purification of triterpene saponins from Clematis mandshurica by high-speed counter-current chromatography coupled with evaporative light scattering detection

Shepo S Shi, Dan D Jiang, Mingbo M Zhao, and Pengfei P Tu

BIOMED APPL 852(1-2):5 (2007) PMID 17442637

Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to the isolation and purification of four triterpene saponins from Clematis mandshurica by one-step separation. The solvent system was composed of ethyl acetate-n-butanol-ethanol-0.05% TFA (5:10:2:20, v/v). The purities of all the saponins obtained were better than 97%. The structures of the compounds were elucidated by the means of spectroscopic methods.

DOI: 10.1016/j.jchromb.2007.02.056
Version: za2963e q8za4 q8zb0 q8zcf q8zda q8ze1 q8zff q8zg8
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