Haematophagous insects incorporate a large amount of blood with each meal, producing a big quantity of urine in a few hours to eliminate the excess water and Na(+). Malpighian tubules (MTs) have traditionally been seen as a system that responds to neuroendocrine stimulus. In a related paper, we demonstrated that MTs of Triatoma infestans produce an autonomous endocrine secretion of an allatotropin-like (AT-like) peptide. In the present study, we report a myostimulatory activity of AT at the level of the hindgut (HG), associated with endocrine mechanisms regulating post-prandial diuresis. Allatotropin induced an increase in frequency and intensity of peristaltic contractions at the level of the HG. The release of the HG content in MTs-HG in vitro preparations undergoing an osmotic shock occurred at different times, depending on the number of MTs present, and there was no release in treatments without MTs. The application of an AT-antiserum to MTs-HG preparations undergoing osmotic shock produced a delay or a long-term blockade of diuresis, depending on the antiserum dilution applied. Similar results were obtained when AT-antiserum was applied in vivo prior to blood intake, decreasing the volume of urine eliminated during the first 2 h. Our results allow us to assign a specific endocrine function to the AT-like peptide released by MTs that is linked to the elimination of urine after blood meals.
We investigate the origin of the diffuse 6.4 keV line emission recently
Detected by Suzaku and the source of H_2ionization in the diffuse molecular gas
Of the Galactic Center (GC) region. We show that Fe atoms and H_2 molecules in
The diffuse interstellar medium of the GC are not ionized by the same...
We demonstrate the movement of a hinged unit supporting the part of the binding groove that interacts with the amino terminal residues of the bound peptide. This unit contains a conserved 3(10) helix that flips from an exposed "open" position in the PR form to a "closed" position in the peptide-load...
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