Advanced search×

Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice

Shigeki S Kato, Yasuaki Y Mohri, Tsuyoshi T Matsuo, Eisaku E Ogawa, Akihiro A Umezawa, Ryuhei R Okuyama, and Katsuhiko K Nishimori

FEBS Lett 581(24):6 (2007) PMID 17850793

We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.

References 29 articles

DOI: 10.1016/j.febslet.2007.08.064
Version: za2963e q8za1 q8zbf q8zcd q8zd6 q8zeb q8zff q8zg8

Similar articles you may find interesting…

  1. Refining the complex rheumatoid arthritis phenotype based on specificity of the HLA-DRB1 shared epitope for antibodies to citrullinated prot...
    Tom W J TW Huizinga, Christopher I CI Amos, ... Lindsey A LA Criswell

    Arthritis Rheum 52(11):3433-8 (2005) PMID 16255021

    For the Leiden EAC, the odds ratio (OR) describing the association of 2 copies of the SE allele with anti-CCP positivity (using no copies of the SE allele in the healthy control group as the referent) was 11.79 (P < 0.0001), while the OR for 1 SE allele was 4.37 (P < 0.0001). No association with the...
  2. Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase
    Takahiro T Suzuki, Shinsuke S Usuda, ... Satoshi S Inouye

    FEBS Lett 581(24):6 (2007) PMID 17825828

    We have applied the method of real-time bioluminescence imaging to investigate protein trafficking in mammalian cells. In the living cells of Chinese hamster ovary and PC12D cells, exocytotic secretion of protein and protein targeting on the cell surface were visualized using the secreted Gaussia lu...
  3. [Effect of aspirin administration for the treatment of osteoporosis in ovariectomized rat model].
    Zhi-Wen Chen, Zi-Xiang Wu, ... Da Zhang

    Zhonghua Yi Xue Za Zhi 91(13):925-9 (2011) PMID 21600123

    Three months after ovariectomy, BMD was significantly lower than preoperative. BMD in aspirin treated groups was significantly higher than that in OVX group. Alkaline phosphatase in peripheral blood decreased significantly in aspirin groups than those in OVX, but osteocalcin had no significant diffe...