A method for indirectly detecting horseradish peroxidase (HRP) was described by CE with electrochemical detection. Details of selection for optimum conditions were presented. The detection limit of free HRP was 1.09 x 10(-12) M or 0.94 zmol (S/N = 3). A novel CE-based liquid-phase binding noncompetitive enzyme immunoassay (CE-EIA) was developed. In this method, after the noncompetitive immunoreaction in liquid phase, the free enzyme (HRP)-labeled antibody (Ab*) and the bound enzyme-labeled complex (Ag-Ab*) were separated and then the system of HRP catalyzing H(2)O(2)/o-aminophenol (OAP) reaction was adopted. Prostate specific antigen (PSA), carcinoembryonic antigen (CEA), and human chorionic gonadotropin (HCG) in human serum samples were detected without any sample preparation, with the detection limits (S/N = 3) of 0.22, 0.17 and 0.30 ng/mL, respectively. This technique has been successfully applied to detect simultaneously PSA, CEA, and HCG in 12 min, upon adding these three antigens into human serum to simulate patient serum. It proves that the CE-EIA technique proposed could be developed into a sensitive and new method for simultaneous clinical assay of multianalytes.
We address the problem of optimal relay placement in a
Cellular network assuming network densification, with the aim of maximizing
Cell capacity. In our model, a fraction of radio resources is dedicated to the
Base-station (BS)/relay nodes (RN) communication. In the remaining resources,
BS and RN tr...
We take a step in
Clarifying this issue, by focusing on a particular example, namely that of a
Two dimensional phase space liquid which emerges when one considers the
Calogero model, a many-body one-dimensional system interacting through an
Inverse square law potential. We demonstrate how the symmet...
We report on the neutron response of the LAMBDA spectrometer developed
Earlier for high-energy gamma-ray measurement. The energy dependent neutron
Detection efficiency of the spectrometer has been measured using the
Time-of-flight (TOF) technique and compared with that of an organic liquid
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