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CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine

Valéria A VA Conforti, David M DM de Avila, Nada S NS Cummings, Ricardo R Zanella, Kenny J KJ Wells, Hasan H Ulker, and Jerry J JJ Reeves

Vaccine 26(7):7 (2008) PMID 18201806

This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n=8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6mg); and one group receiving 3.4mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. All vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization.

DOI: 10.1016/j.vaccine.2007.12.010
Version: za2963e q8zad q8zba q8zcf q8zd6 q8zeb q8zfe q8zg0

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