Lamin B1, a major component of the nuclear lamina, anchors the nucleus to the cytoskeletal cage, and controls nuclear orientation, chromosome positioning and, alongside several enzymes, fundamental nuclear functions. Exposing polyomavirus-transformed rat pyF111 fibroblasts and human cervical carcinoma (HCC) C4-I cells for 30 min to photoexcited perylenequinone calphostin C, i.e. Cal C^@f^E, an established reactive oxygen species (ROS)-generator and protein kinase C (PKC) inhibitor, caused the cells to selectively oxidize and then totally destroy their nuclear lamin B1 by only 60 min after starting the treatment, i.e. when apoptotic caspases' activities had not yet increased. However, while the oxidized lamin B1 was being destroyed, lamins A/C, the lamin A-associated nuclear envelope protein emerin, and the nucleoplasmic protein cyclin E were neither oxidized nor destroyed. The oxidized lamin B was ubiquitinated and demolished in the proteasome probably by an enhanced peptidyl-glutaminase-like activity. Hence, the Cal C^@f^E-induced rapid and selective lamin B1 oxidation and proteasomal destruction ahead of the activation of apoptotic caspases was by itself a most severe molecular lesion impairing vital nuclear functions. Conversely, Cal C directly added to the cells kept in the dark damaged neither nuclear lamin B1 nor cell viability. Thus, our findings reveal a novel cell-damaging mechanism of a photodynamic tumor therapeutic agent.
Weak measurements can be seen as an attempt at answering the 'Which way?'
question without destroying interference between the pathways involved. Unusual
mean values obtained in such measurements represent the response of a quantum
system to this 'forbidden' question, in which the 'true' compositio...
We develop an
Integrated approach that exploits the representation redundancy inherent in the
Multiple streamed videos a voxel in the 3D scene visible to two captured views
Is sampled and coded twice in the two views. In particular, at the receiver we
First develop an error concealment strategy that...
We generate nonclassical conditional states by exploiting the quantum
Correlations of multimode twin-beam states obtained in the mesoscopic
Photon-number regime. A strong relation between the sub-shot-noise correlations
In twin beams and sub-Poissonian character of the conditional states is
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