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Substance P increases production of proinflammatory cytokines and formation of osteoclasts in dental pulp fibroblasts in patients with severe orthodontic root resorption

Masaru M Yamaguchi, Yasuhito Y Ozawa, Hiroyuki H Mishima, Norihito N Aihara, Tadashi T Kojima, and Kazutaka K Kasai

Am J Orthod Dentofacial Orthop 133(5):9 (2008) PMID 18456142

Introduction: The objective of this study was to determine the extent to which substance P (SP) increases proinflammatory cytokine production and osteoclast formation of human dental pulp fibroblasts (HDPF) in patients with severe orthodontically induced inflammatory root resorption (OIIRR). Methods: HDPF were obtained from 5 patients with severe apical root resorption after orthodontic treatment. The levels of interleukin (IL)-1@b, IL-6, and tumor necrosis factor (TNF)-@a were determined after 24 hours by using ELISA kits. Furthermore, culture supernatants were added to cultured human osteoclasts, and osteoclast formation was observed after tartrate-resistant acid phosphatase (TRAP) staining and the formation of resorption cavities. Results: Stimulation with SP increased the levels of IL-1@b, IL-6, and TNF-@a, in a time- and concentration-dependent manner, although the increase was greater in the severe root resorption (SRR) group than in the nonresorption (NR) group (P <0.001, 2-way repeated measures ANOVA). Conclusions: These results suggest that HDPF stimulated with SP might be deeply involved in the progress of inflammation in pulp tissue and the incidence of SRR during orthodontic treatment.

Copyright © 2008 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.ajodo.2006.03.043
Version: za2963e q8za2 q8zb5 q8zc9 q8zd9 q8zec q8zf0 q8zg8
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