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Normal reproductive capacity of heifers that originated from in vitro fertilized embryos cultured with an antiviral compound

M Daniel MD Givens, Mylissa S D MS Marley, Kay P KP Riddell, Patricia K PK Galik, and David A DA Stringfellow

Anim Reprod Sci 113(1-4):4 (2009) PMID 18691836

Bovine viral diarrhea virus (BVDV) can associate with in vitro fertilized (IVF) bovine embryos despite washing and trypsin treatment. An antiviral compound, DB606 (2-(4-[2-imidazolinyl]phenyl)-5-(4-methoxyphenyl)furan), inhibits the replication of BVDV in bovine uterine tubal epithelial cells, Madin Darby bovine kidney cells, and fetal fibroblast cells. As well, DB606 in in vitro culture medium does not affect embryonic development. Antiviral-treated-IVF embryos placed into recipients developed into clinically normal calves. The objective of this project was to determine if these resultant heifer calves were capable of reproducing. Seven heifers from each of the treatment groups (natural breeding, IVF embryo, and IVF embryo cultured in DB606) of the previous study were used. At 20-27months of age, the heifers were exposed to a fertile bull in a single pasture during a 63d breeding season. Five of the seven heifers originating from natural breeding were pregnant 35d after removal of the bull and calved. All of the heifers resulting from transfer of untreated IVF embryos were pregnant at 35d; however, one aborted the fetus at 5-7months of gestation. All of the heifers derived from transfer of IVF embryos cultured in DB606 were pregnant and calved. Offspring from dams of all treatment groups were clinically normal at birth. Adjusted 205d weaning weights were not significantly different among the offspring of the treated and untreated dams. These results indicate that culture of bovine-IVF embryos in DB606 does not impair future reproductive capacity of resulting heifers.

Copyright © 2009 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.anireprosci.2008.06.010
Version: za2963e q8za1 q8zb0 q8zc1 q8zd1 q8ze6 q8zf2 q8zg1

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