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Interaction of grape ASR proteins with a DREB transcription factor in the nucleus

Amélie A Saumonneau, Alice A Agasse, Marie-Thérèse MT Bidoyen, Magali M Lallemand, Anne A Cantereau, Anna A Medici, Maryse M Laloi, and Rossitza R Atanassova

FEBS Lett 582(23-24):7 (2008) PMID 18804467

ASR proteins (abscissic acid, stress, ripening induced) are involved in plant responses to developmental and environmental signals but their biological functions remain to be elucidated. Grape ASR gene (VvMSA) encodes a new transcription factor regulating the expression of a glucose transporter. Here, we provide evidence for some polymorphism of grape ASRs and their identification as chromosomal non-histone proteins. By the yeast two-hybrid approach, a protein partner of VvMSA is isolated and characterized as an APETALA2 domain transcription factor. Interaction of the two proteins is further demonstrated by the BiFC approach and the exclusive nuclear localization of the heterodimer is visualized. Structured summary: MINT-6743067: VvMSA (uniprotkb:Q94G23) and VvDREB (uniprotkb:A6XA90) physically interact (MI:0218) by bimolecular fluorescence complementation (MI:0809) MINT-6743043: VvMSA (uniprotkb:Q94G23) physically interacts (MI:0218) with VvDREB (uniprotkb:A6XA90) by two hybrid (MI:0018)

DOI: 10.1016/j.febslet.2008.09.015
Version: za2963e q8za5 q8zbe q8zcd q8zd7 q8zee q8zfd q8zg9
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