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The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth.

U U Salma, M S M MS Rahman, S S Islam, N N Haque, T A TA Jubair, A K M F AK Haque, and I J IJ Mukti

Pak J Biol Sci 11(12):1638-41 (2008) PMID 18819656

The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and 2.0 mg L(-1) NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L(-1) BA with 0.2 mg L(-1) NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L(-1) each of IBA and IAA.

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