In this experimental work we employed single-molecule electrical recordings on alamethicin oligomers inserted in lipid bilayers made of brain sphingomyelin (bSM), palmitoyloleoylphosphatidylcholine (POPC) and cholesterol (chol) to unravel novel aspects regarding lipid raft interactions with pore-forming peptides. We probed the effect of lipid rafts on electrical properties of inserted alamethicin oligomers, and our data convincingly prove that the single-channel electrical conductance of various subconductance states of the alamethicin oligomer (1) increases in the presence of raft-containing ternary lipid mixtures (POPC-chol-bSM) compared to cases when bilayers were made of POPC-chol and POPC and (2) decreases in the presence of raft-containing ternary lipid mixtures compared to nonraft ternary mixtures which favor the fluid and liquid ordered phases alone. Our data demonstrate that the presence of lipid rafts leads to a slower association kinetics of alamethicin oligomers, seemingly reflecting a slower lateral diffusion process of such peptide aggregates compared to the case of nonraft, binary lipid mixtures. Furthermore, we show that the electrical capacitance of ternary lipid mixtures (POPC-chol-bSM) decreases in the presence of raft domains by comparison to nonraft binary phases (POPC-chol) or POPC alone, and this could constitute an additional mechanism via which macroscopic electrical manifestations of eukaryotic cells are modulated by the coexistence of gel and fluid domains of the plasma membrane.
We consider voltage-driving DNA translocation through a nanopore in the
Present study. By assuming the DNA is coaxial with the cylindrical nanopore, a
Hydrodynamic model for determining effective force on a single DNA molecule in
A nanopore was presented, in which density functional theory (DFT) com...
We identify and investigate a computational model arising in molecular
Computing, social computing and sensor network. The model is made of of
Multiple agents who are computationally limited and posses no global
Information. The agents may represent nodes in a social network, sensors, or
We investigate the role of thermal
Pressure support in hypermassive merger remnants by computing sequences of
Spherically-symmetric and axisymmetric uniformly and differentially rotating
Equilibrium solutions to the general-relativistic stellar structure equations.
Using a set of finite-temperature...
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