Here we present the first diploid genome sequence of an Asian individual. The genome was sequenced to 36-fold average coverage using massively parallel sequencing technology. We aligned the short reads onto the NCBI human reference genome to 99.97% coverage, and guided by the reference genome, we used uniquely mapped reads to assemble a high-quality consensus sequence for 92% of the Asian individual's genome. We identified approximately 3 million single-nucleotide polymorphisms (SNPs) inside this region, of which 13.6% were not in the dbSNP database. Genotyping analysis showed that SNP identification had high accuracy and consistency, indicating the high sequence quality of this assembly. We also carried out heterozygote phasing and haplotype prediction against HapMap CHB and JPT haplotypes (Chinese and Japanese, respectively), sequence comparison with the two available individual genomes (J. D. Watson and J. C. Venter), and structural variation identification. These variations were considered for their potential biological impact. Our sequence data and analyses demonstrate the potential usefulness of next-generation sequencing technologies for personal genomics.
We systematically overexpressed 99 transcription factor genes with the nmt1 promoter and found that 64 transcription factor genes exhibited reduced fitness when ectopically expressed. Cell cycle defects were also often observed. We further investigated three uncharacterized transcription factor gene...
We give the first example of a transitive quadratic map whose real and
Complex geometric pressure functions have a high-order phase transition. In
Fact, near the phase transition these functions behave as $x \mapsto \exp (- 1
/ x^2)$ near $x = 0$, before becoming linear. This quadratic map has a
We analyse a previously proposed cell-based model of
Glioblastoma (brain tumour) growth, which is based on the assumption that the
Cancer cells switch phenotypes between a proliferative and motile state (Gerlee
And Nelander, PLoS Comp. Bio., 8(6) 2012). The dynamics of this model can be
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