Advanced search×

Subcellular localization and in vivo oxidation-reduction kinetics of thiol peroxidase in Escherichia coli.

Kazuyuki K Tao

FEMS Microbiol Lett 289(1):41-5 (2008) PMID 19054092

Peroxiredoxins are a class of peroxide-scavenging enzymes having a conserved cysteine residue(s) in their active centers. Thiol peroxidase (Tpx) is one of the peroxiredoxins identified in Escherichia coli. Despite the absence of the N-terminal signal sequence for transport across the membrane, it has been characterized as a periplasmic protein. Reanalysis of Tpx localization, using active site cysteine mutants of thioredoxin 1 (Trx1), demonstrated that Tpx forms a mixed-disulfide complex with cytoplasmic Trx1, indicating that Tpx localizes in the cytoplasm.

DOI: 10.1111/j.1574-6968.2008.01372.x
Version: za2963e q8zac q8zb8 q8zc4 q8zd7 q8ze6 q8zf5 q8zg5
Buy PDF

Similar articles you may find interesting…

  1. Solution-dispersible Au nanocube dimers with greatly enhanced two-photon luminescence and SERS.
    Xiao-Li Liu, Shan Liang, and Qu-Quan Wang

    Nanoscale (2013) PMID 23649164

    We report the synthesis of 43-nm diameter Au nanocube dimers by using Ag(+) ions as competitive ligands to freeze l-cysteine-induced assembly process of the nanocubes to a desirable stage. Ascribed to the resonant interparticle coupling with an newly arising plasmon band at 710 nm and local field en...
    Buy PDF
  2. Transcription Factor NF-E2-Related Factor 2 (Nrf2)-Mediated Antioxidant Defense System in the Development of Diabetic Retinopathy.
    Qing Zhong, Manish Mishra, and Renu A Kowluru

    Invest Ophthalmol Vis Sci (2013) PMID 23633659

    Due to increased binding of Nrf2 with Keap1, its translocation to the nucleus is compromised contributing to the decreased GSH levels. Thus, regulation of Nrf2-Keap1 by pharmacological or molecular means could serve as a potential adjunct therapy to combat oxidative stress and inhibit the developmen...
    Buy PDF
  3. The C-terminal region of Rad52 is essential for Rad52 nuclear and nucleolar localization, and accumulation at DNA damage sites immediately a...
    Manabu Koike, Yasutomo Yutoku, and Aki Koike

    Biochem Biophys Res Commun (2013) PMID 23639616

    We show that the subcellular localization of EYFP-Rad52(1-418) changes dynamically during the cell cycle. In addition, EYFP-Rad52(1-418) accumulates rapidly at microirradiated sites and colocalizes with the DSB sensor protein Ku80. Moreover, the accumulation of EYFP-Rad52(1-418) at DSB sites is inde...
    Buy PDF