Two-photon (2P) microscopy has become increasingly popular among immunologists for analysing single-cell dynamics in tissues. Researchers are now taking 2P microscopy beyond the study of model antigen systems (e.g. ovalbumin immunization) and are applying the technique to examine infection in vivo. With the appropriate fluorescent probes, 2P imaging can provide high-resolution spatio-temporal information regarding cell behaviour, monitor cell functions and assess various outcomes of infection, such as host cell apoptosis or pathogen proliferation. Imaging of transgenic and knockout mice can be used to probe molecular mechanisms governing the host response to infection. From the microbe side, imaging genetically engineered mutant strains of a pathogen can test the roles of specific virulence factors in pathogenesis. Here, we discuss recent work that has applied 2P microscopy to study models of infection and highlight the tremendous potential that this approach has for investigating host-pathogen interactions.
We report the symptomatology, the imaging and discuss the different surgical managements. We conclude that the clinician must have a high index of suspicion when dealing with a unilateral rhinosinusitis persisting despite a maximal and well conducted medical treatment. This is particularly so in eld...
We demonstrate a compact, robust, and highly efficient source of
Polarization-entangled photons, based on linear bi-directional down-conversion
In a novel 'folded sandwich' configuration. Bi-directionally pumping a single
Periodically poled KTiOPO$_4$ (ppKTP) crystal with a 405-nm laser diode, we
We study distribution schemes for a polarization entangled photon pair based
On a decoherence-free subspace over lossy quantum channels and propose an
Efficient scheme that is robust against not only collective phase noises but
Also general collective noises for two qubits. While the proposed scheme...
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