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Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

Malena E Sandberg, Denise Schellmann, Gerda Brunhofer, Thomas Erker, Igor Busygin, Reko Leino, Pia M Vuorela, and Adyary Fallarero

J Microbiol Methods 78(1):3 (2009) PMID 19427338

Staining of Staphylococcus aureus biofilms with 20 @?M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated.

Copyright © 2009 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.mimet.2009.04.014
Version: za2963e q8zab q8zba q8zc9 q8zda q8ze0 q8zf8 q8zg7
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