Advanced search×

Antihypertensive effects of flavonoids isolated from brazilian green propolis in spontaneously hypertensive rats.

Hiroe Maruyama, Yoshiki Sumitou, Takashi Sakamoto, Yoko Araki, and Hideaki Hara

Biol Pharm Bull 32(7):1244-50 (2009) PMID 19571393

Propolis, a honeybee product, has become popular as a food and alternative medicine. Its constituents have been shown to exert pharmacological effects, such as anticancer, antimicrobial, and anti-inflammatory effects. The present study was performed to investigate whether Brazilian green propolis exerts antihypertensive effects in spontaneously hypertensive rats (SHR) and which constituents are involved in its effects. Brazilian green propolis was extracted with ethanol and subjected to LH-20 column chromatography eluted with ethanol. The ethanol-eluted fractions at 10 mg/kg were administered orally to SHR for 14 d. Significant decreases in blood pressure were observed in fractions 6 and 7. The active constituents were purified and identified to be four flavonoids: dihydrokaempferide and isosakuranetin in fraction 6 and betuletol and kaempferide in fraction 7. These flavonoids at 10 mg/kg were administered orally to SHR for 28 d, and as a result, isosakuranetin, dihydrokaempferide and betuletol produced significant decrease in blood pressure, especially marked were the effects observed in the group that received isosakuranetin. Brazilian green propolis, fractions 6 and 7, and the 4 active constituents relaxed isolated SHR aorta in a concentration-dependent manner. Therefore, these finding suggest that the vasodilating action may be partly involved in the mechanism of antihypertensive effect. Hence, the ethanol extract of Brazilian green propolis and its main constituents may be useful for prevention of hypertension.

DOI: 10.1248/bpb.32.1244
Version: za2963e q8za0 q8zb5 q8zcd q8zd3 q8ze0 q8zf7 q8zg3

Similar articles you may find interesting…

  1. Bee products prevent VEGF-induced angiogenesis in human umbilical vein endothelial cells.
    Hiroshi H Izuta, Masamitsu M Shimazawa, ... Hideaki H Hara

    Audio, Transactions of the IRE Professional Gro... (2009) PMID 19917137

    Among the bee products, Chinese red propolis and CAPE in particular showed strong suppressive effects against VEGF-induced angiogenesis. These findings indicate that Chinese red propolis and CAPE may have potential as preventive and therapeutic agents against angiogenesis-related human diseases....
  2. Different extraction methods of biologically active components from propolis: a preliminary study.
    Boryana B Trusheva, Dorina D Trunkova, and Vassya V Bankova

    Audio, Transactions of the IRE Professional Gro... (2007) PMID 17880743 PMCID 1994058

    The total amounts of extracted phenolics and flavonoids were determined, and the effectiveness of the methods compared. MAE was very rapid but led to the extraction of a large amount of non-phenolic and non-flavonoid material. UE gave the highest percentage of extracted phenolics. CONCLUSION: Compar...
    View PDF
  3. A novel member of the RNase D exoribonuclease family functions in mitochondrial guide RNA metabolism in Trypanosoma brucei.
    Sara L Zimmer, Sarah M McEvoy, ... Laurie K Read

    J Biol Chem 286(12):10329-40 (2011) PMID 21252235 PMCID PMC3060487

    We have identified a mitochondrial exoribonuclease, TbRND, whose expression is highly upregulated in the insect proliferative stage of the parasite. TbRND shares sequence similarity with RNase D family enzymes, but differs from all reported members of this family in possessing a CCHC zinc finger dom...
    PDF not found