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Phage display selection, analysis, and prediction of B cell epitopes.

Natalia Tarnovitski NT Freund, David D Enshell-Seijffers, and Jonathan M JM Gershoni

Curr Protoc Immunol (2009) PMID 19653209

Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided.

DOI: 10.1002/0471142735.im0908s86
Version: za2963e q8za8 q8zb5 q8zc0 q8zdb q8ze4 q8zfc q8zgf

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