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Quantitative TaqMan real-time PCR assays for gene expression normalisation in feline tissues.

Yvonne Y Kessler, A Katrin AK Helfer-Hungerbuehler, Valentino V Cattori, Marina L ML Meli, Bigna B Zellweger, Pete P Ossent, Barbara B Riond, Claudia E CE Reusch, Hans H Lutz, and Regina R Hofmann-Lehmann

BMC Mol Biol (2009) PMID 20003366

Gene expression analysis is an important tool in contemporary research, with real-time PCR as the method of choice for quantifying transcription levels. Co-analysis of suitable reference genes is crucial for accurate expression normalisation. Reference gene expression may vary, e.g., among species or tissues; thus, candidate genes must be tested prior to use in expression studies. The domestic cat is an important study subject in both medical research and veterinary medicine. The aim of the present study was to develop TaqMan real-time PCR assays for eight potential reference genes and to test their applicability for feline samples, including blood, lymphoid, endocrine, and gastrointestinal tissues from healthy cats, and neoplastic tissues from FeLV-infected cats.

DOI: 10.1186/1471-2199-10-106
Version: za2963e q8za2 q8zb8 q8zce q8zdc q8ze3 q8zfb q8zg7
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