Advanced search×

Isolation of mitochondria for biogenetical studies: An update

Erika E Fernández-Vizarra, Gustavo G Ferrín, Acisclo A Pérez-Martos, Patricio P Fernández-Silva, Massimo M Zeviani, and José Antonio JA Enríquez

Mitochondrion 10(3):10 (2010) PMID 20034597

The use of good quality preparations of isolated mitochondria is necessary when studying the mitochondrial biogenetical activities. This article explains a fast and simple method for the purification of mammalian mitochondria from different tissues and cultured cells, that is suitable for the analysis of many aspects of the organelle's biogenesis. The mitochondria isolated following the protocol described here, are highly active and capable of DNA, RNA and protein synthesis. Mitochondrial tRNA aminoacylation, mtDNA-protein interactions and specific import of added proteins into the organelles, can also be studied using this kind of preparations.

Copyright © 2010 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.mito.2009.12.148
Version: za2963e q8za9 q8zba q8zca q8zda q8ze4 q8zf8 q8zg4

Similar articles you may find interesting…

  1. Hereditary cataract in the Welsh Springer spaniel.
    K C Barnett

    J Small Anim Pract 21(11):621-5 (1980) PMID 7230748

  2. From the World Brain to the First Transatlantic Information Dialogue: activities in information and documentation in Germany in the first ha...
    Thomas Hapke

    IFLA Journal 29(4):364-377 (2003)

  3. Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells.
    Erika Fernández-Vizarra, Manuel J López-Pérez, and José A Enriquez

    Methods 26(4):292-7 (2002) PMID 12054919

    This article describes a quick basic method adapted for the purification of mammalian mitochondria from different sources. The organelles obtained using this protocol are suitable for the investigation of biogenetic activities such as enzyme activity, mtDNA, mtRNA, mitochondrial prot...