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Candida albicans biofilm formation on peptide functionalized polydimethylsiloxane.

Kristof K De Prijck, Nele N De Smet, Monika M Rymarczyk-Machal, Gonzalez G Van Driessche, Bart B Devreese, Tom T Coenye, Etienne E Schacht, and Hans J HJ Nelis

Biofouling 26(3):269-75 (2010) PMID 20054722

In order to prevent biofilm formation by Candida albicans, several cationic peptides were covalently bound to polydimethylsiloxane (PDMS). The salivary peptide histatin 5 and two synthetic variants (Dhvar 4 and Dhvar 5) were used to prepare peptide functionalized PDMS using 4-azido-2,3,5,6-tetrafluoro-benzoic acid (AFB) as an interlinkage molecule. In addition, polylysine-, polyarginine-, and polyhistidine-PDMS surfaces were prepared. Dhvar 4 functionalized PDMS yielded the highest reduction of the number of C. albicans biofilm cells in the Modified Robbins Device. Amino acid analysis demonstrated that the amount of peptide immobilized on the modified disks was in the nanomole range. Poly-d-lysine PDMS, in particular the homopeptides with low molecular weight (2500 and 9600) showed the highest activity against C. albicans biofilms, with reductions of 93% and 91%, respectively. The results indicate that the reductions are peptide dependent.

DOI: 10.1080/08927010903501908
Version: za2963e q8za7 q8zb9 q8zce q8zd1 q8ze6 q8zfb q8zg2

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