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Clinical profiling and use of loop-mediated isothermal amplification assay for rapid detection of Mycobacterium tuberculosis from sputum.

A A Poudel, B D BD Pandey, B B Lekhak, B B Rijal, B R BR Sapkota, and Y Y Suzuki

Kathmandu Univ Med J (KUMJ) 7(26):109-14 (2009) PMID 20071841

Tuberculosis is a global health problem and the situation is worsening with newer incidences of drug resistance and HIV association. Diagnosis of tuberculosis can be done by many methods and test, culture of sputum being the ideal one. Nucleic acid amplification (NAA) assay are more time efficient one, that amplify and detect specific nucleic acid sequences allows rapid, sensitive and specific detection of M. tuberculosis in sputum samples.

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