Advanced search×

Latest developments in sample treatment for ^1^8O-isotopic labeling for proteomics mass spectrometry-based approaches: A critical review

JL Capelo, RJ Carreira, L Fernandes, C Lodeiro, HM Santos, and J Simal-Gandara

Talanta 80(4):11 (2010) PMID 20082805

Nowadays isotopic ^1^8O-labeling of peptides has recalled the attention of researchers due to its simplicity of application and high versatility for proteomics studies. Protein quantification, differential peptide mass mapping, studies regarding proteins overexpressed or underexpressed, or the searching of biomarkers can be accomplished by using ^1^8O-labeling. In this critical review we comment on the different ways in which ^1^8O-labeling can be done, highlighting the key parameters of the different sample treatments to obtain a reliable and reproducible labeling. In addition we describe and compare the latest improvement in terms of sample treatment that allows to reduce the handling and to increase the throughput for this sample treatment. Finally, we hypothesize on the future trends of these methods under the light of the new technological advances to speed protein cleavage.

Copyright © 2010 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.talanta.2009.04.053
Version: za2963e q8za5 q8zb4 q8zcd q8zdd q8ze9 q8zfa q8zgd
Buy PDF

Similar articles you may find interesting…

  1. Imidafenacin for the treatment of overactive bladder.
    Umberto Leone Roberti Maggiore, Carolina Scala, and Simone Ferrero

    Expert Opin Pharmacother (2013) PMID 23641864

    Imidafenacin (KRP-197/ONO-8025) is the latest antimuscarinic (AM) developed for the treatment of overactive bladder syndrome (OAB) and, at the moment, it is marketed only in Japan. This compound has been developed to improve the tolerability of AM therapy by binding specifically the M3 receptor subt...
    Buy PDF
  2. Pubertal status of children and adolescents during orthodontic treatment.
    J Hoffmann, K-F Krey, and C Hirsch

    J Orofac Orthop (2013) PMID 23649281

    The orthodontic subgroup included 296 boys and girls (accounting for 29.3% of the total sample), 50% of whom (n=148) had already attained advanced stages of pubertal development. Broken down by gender, we observed advanced stages in 60% of the girls versus 38% of the boys (p<0.001). However, this ge...
    Buy PDF
  3. Reducing Sequence Artifacts in Amplicon-Based Massively Parallel Sequencing of Formalin-Fixed Paraffin-Embedded DNA by Enzymatic Depletion o...
    Hongdo Do, Stephen Q Wong, and Alexander Dobrovic

    Clin Chem (2013) PMID 23649127

    UDG pretreatment greatly facilitates the accurate discrimination of mutations in FFPE samples by use of amplicon-based approaches. This is particularly important when working with samples with low tumor purity or for the assessment of mutational heterogeneity in tumors....
    Buy PDF