Advanced search×

Enhanced thermal and ultrasonic stability of a fungal protease encapsulated within biomimetically generated silicate nanospheres

Ashkan Madadlou, Daniela Iacopino, David Sheehan, Zahra Emam-Djomeh, and Mohammad E Mousavi

General Subjects 1800(4):7 (2010) PMID 20100547

Background: Dendrimers are highly branched synthetic macromolecules with a globular shape. They have been successfully used for generation of nanospheres at mild conditions via biomimetic silicification. Encapsulation of enzyme molecules within these nanospheres during their synthesis is a promising method for rapid and efficient entrapment of several enzymes. However, encapsulation of proteolytic enzymes has been rarely done via biomimetic silicification. As well, the operational stability of encapsulated enzyme has not been systematically reported. Methods: A proteolytic enzyme, either @a-Chymotrypsin or a fungal protease from Aspergilus Oryzea was encapsulated along with iron oxide nanoparticles within particles yielded via biomimetic silicification of different generations of polyamidoamine (PAMAM) dendrimers. Stability of encapsulated enzyme was compared to that of free enzyme during storage at room temperature. As well, their thermal and ultrasonic stabilities were measured. Scanning electron microscopy, transmission electron microscopy and optical microscopy were used to investigate the morphology of nanospheres. Results: Determination of encapsulation efficiency revealed that ~85% of fungal protease with concentration 1.4mg mL^-^1 stock solution was immobilized within particles yielded by generation 0. Based on microscopic images the generated particles interconnected with each other and had spherical morphologies independent of generation. Kinetic analysis of encapsulated fungal protease demonstrated that Mechaelis-Menten constant (K"m) slightly increased. Conclusion: PAMAM dendrimer generation 0 could be effectively used for rapid encapsulation of a fungal protease from Aspegilus Oryzae. General significance: Encapsulation significantly enhances the thermal and ultrasonic stabilities of enzymes, suggesting a range of diverse applications for them.

DOI: 10.1016/j.bbagen.2010.01.004
Version: za2963e q8za6 q8zba q8zca q8zdb q8ze5 q8zfd q8zgf

Similar articles you may find interesting…

  1. Rheology, microstructure, and functionality of low-fat Iranian white cheese made with different concentrations of rennet.
    A Madadlou, A Khosroshahi, and M E Mousavi

    J Dairy Sci 88(9):3052-62 (2005) PMID 16107393

    A batch of full-fat (23% target fat) and 3 batches of low-fat (6% target fat) Iranian white cheese with different rennet concentrations (1-, 2-, and 3-fold the normal usage) were produced to study the effect of fat content reduction and promoted proteolysis on the textural and functional properties...
  2. Level of adherence to the Mediterranean diet among children from Cyprus: the CYKIDS study.
    Chrystalleni Lazarou, Demosthenes B Panagiotakos, and Antonia-Leda Matalas

    Public Health Nutr 12(7):991-1000 (2009) PMID 18752695

    Only 6.7% of the sample was classified as high adherers of MD, whereas 37% had a poor KIDMED score. Multiple logistic regression analysis has shown that children with at least an average KIDMED score were more likely to eat frequently seafood and fish, legumes, nuts, bread, fruits, leafy vegetables,...
  3. Randomized, controlled clinical trial of zinc supplementation to prevent immunological failure in HIV-infected adults.
    Marianna K MK Baum, Shenghan S Lai, ... Adriana A Campa

    Clin Infect Dis 50(12):1653-60 (2010) PMID 20455705

    This study demonstrated that long-term (18-month) zinc supplementation at nutritional levels delayed immunological failure and decreased diarrhea over time. This evidence supports the use of zinc supplementation as an adjunct therapy for HIV-infected adult cohorts with poor viral control. Trial regi...
    View PDF