A one-step real-time Taqman^(R) RT-PCR assay (RRT-PCR) for peste des petits ruminants virus (PPRV) was developed to detect the four lineages of PPRV by targeting the nucleoprotein (N) gene of the virus. This new assay was compared to a conventional RT-PCR on reference strains and field materials. Quantitation was performed against a standard based on a synthetic transcript of the NPPR gene for which a minimum of 32 copies per reaction were detected with a corresponding C"t value of 39. Depending on the lineage involved, the detection limit of RRT-PCR was decreased by one to three log copies relative to the conventional method. The lower stringency occurred with lineage III because of minor nucleotide mismatches within the probe region. The assay did not detect phylogenetically or symptomatically related viruses of ruminants (such as rinderpest, bluetongue, and bovine viral diarrhea viruses). However, it was capable of detecting 20% more positive field samples with low viral RNA loads compared to the conventional PCR method. When compared on a proficiency panel to the method developed by Bao et al. (2008), the sensitivity of the in-house assay was slightly improved on lineage II. It proved significantly faster to perform and hence better adapted for monitoring large numbers of at risk or diseased animals.
We engineered CHA be thermostable from 37 °C to 60 °C and in consequence have generalized its application to real-time detection of isothermal amplification reactions. CHA circuits were designed and optimized for both high and low temperature rolling circle amplification (RCA) and strand displacem...
We study supervisor localization for real-time discrete-event systems (DES)
In the Brandin-Wonham framework of timed supervisory control. We view a
Real-time DES as comprised of asynchronous agents which are coupled through
Imposed logical and temporal specifications; the essence of supervisor
We review recent theoretical and experimental e?orts aimed at the
Investigation of the physics of interacting disordered bosons (so-called dirty
Bosons) in the context of quantum magnetism. The physics of dirty bosons is
Relevant to a wide variety of condensed matter systems, encompassing Helium in...
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