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Functional reconstitution into liposomes of purified human RhCG ammonia channel.

Isabelle I Mouro-Chanteloup, Sylvie S Cochet, Mohamed M Chami, Sandrine S Genetet, Nedjma N Zidi-Yahiaoui, Andreas A Engel, Yves Y Colin, Olivier O Bertrand, and Pierre P Ripoche

PLoS One 5(1):e8921 (2010) PMID 20126667

Rh glycoproteins (RhAG, RhBG, RhCG) are members of the Amt/Mep/Rh family which facilitate movement of ammonium across plasma membranes. Changes in ammonium transport activity following expression of Rh glycoproteins have been described in different heterologous systems such as yeasts, oocytes and eukaryotic cell lines. However, in these complex systems, a potential contribution of endogenous proteins to this function cannot be excluded. To demonstrate that Rh glycoproteins by themselves transport NH(3), human RhCG was purified to homogeneity and reconstituted into liposomes, giving new insights into its channel functional properties.

DOI: 10.1371/journal.pone.0008921
Version: za2963e q8za9 q8zbb q8zce q8zdf q8ze4 q8zfa q8zg7
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