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Cryo-EM model of the bullet-shaped vesicular stomatitis virus.

Peng P Ge, Jun J Tsao, Stan S Schein, Todd J TJ Green, Ming M Luo, and Z Hong ZH Zhou

Science 327(5966):689-93 (2010) PMID 20133572

Vesicular stomatitis virus (VSV) is a bullet-shaped rhabdovirus and a model system of negative-strand RNA viruses. Through direct visualization by means of cryo-electron microscopy, we show that each virion contains two nested, left-handed helices: an outer helix of matrix protein M and an inner helix of nucleoprotein N and RNA. M has a hub domain with four contact sites that link to neighboring M and N subunits, providing rigidity by clamping adjacent turns of the nucleocapsid. Side-by-side interactions between neighboring N subunits are critical for the nucleocapsid to form a bullet shape, and structure-based mutagenesis results support this description. Together, our data suggest a mechanism of VSV assembly in which the nucleocapsid spirals from the tip to become the helical trunk, both subsequently framed and rigidified by the M layer.

DOI: 10.1126/science.1181766
Version: za2963e q8za7 q8zbe q8zcf q8zd9 q8ze4 q8zfe q8zg2
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