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PURE technology.

Yoshihiro Shimizu and Takuya Ueda

Methods Mol Biol (2010) PMID 20204844

The Escherichia coli-based reconstituted cell-free protein synthesis system, which we named the PURE (Protein synthesis Using Recombinant Elements) system, provides several advantages compared with the conventional cell-extract-based system. Stability of RNA or protein is highly improved because of the lack of harmful degradation enzymes. The system can be easily engineered according to purposes or the proteins to be synthesized, by manipulating the components in the system. In this chapter, we describe the construction and exploitation of the PURE system. Methods for preparing and assembling the components composing the PURE system for the protein synthesis reaction are shown.

DOI: 10.1007/978-1-60327-331-2_2
Version: za2963e q8zae q8zb0 q8zc7 q8zd5 q8ze0 q8zfd q8zg1
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