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Peek a peak: a glance at statistics for quantitative label-free proteomics.

Podwojski K, Eisenacher M, Kohl M, Turewicz M, Meyer HE, Rahnenfuhrer J, and Stephan C

Audio, Transactions of the IRE Professiona... 7(2):249-61 (2010) PMID 20377391

Today, label-free mass spectrometry methods are frequently used for quantification of proteins and peptides. There have been several proposals of measurable parameters that best reflect quantities, such as peak areas as well as spectral counts. This review provides a systematic overview of the proposed methods. Owing to the shotgun proteomics approach generally used today for label-free mass spectrometry, any quantitative measure in the first place is a measure of peptide quantity. There has been no systematic research on how to best infer protein quantity from its measured peptides' quantities. The way peptide identifications are assembled to protein lists may especially lead to significantly different results in protein quantification. A further focus of this review will thus be the assembly of measured peptide quantities to a protein quantity.

DOI: 10.1586/epr.09.107
Version: za2963e q8zab q8zb0 q8zc3 q8zdb q8zef q8zfc q8zg3

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