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Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas

Celia Vazquez-Boucard, Pindaro Alvarez-Ruiz, Cristina Escobedo-Fregoso, Gerardo Anguiano-Vega, Ma de Jesus Duran-Avelar, Vania Serrano Pinto, and César Marcial Escobedo-Bonilla

J Invertebr Pathol 104(3):3 (2010) PMID 20398672

Oysters Crassostrea gigas were placed at water supply canals of three shrimp farms in Guasave, Mexico where WSSV outbreaks occur. Animals were sampled through April-August and September-December to detect WSSV DNA. By using three different PCR protocols, only oysters from a farm undergoing a WSSV outbreak were found WSSV-positive in gills and digestive gland. Two WSSV amplicons were sequenced and they corresponded over 99% to WSSV genome segments. Results showed that oysters can capture WSSV particles suspended in water. Susceptibility of oysters to WSSV infection and their role as a carrier remain to be determined.

Copyright © 2010 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.jip.2010.04.004
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