Advanced search×

Lettuce infectious yellows virus (LIYV) RNA 1-encoded P34 is an RNA-binding protein and exhibits perinuclear localization

Jinbo Wang, Lucy R Stewart, Zsofia Kiss, and Bryce W Falk

Virology 403(1):11 (2010) PMID 20447670

The Crinivirus, Lettuce infectious yellows virus (LIYV) has a bipartite, positive-sense ssRNA genome. LIYV RNA 1 encodes replication-associated proteins while RNA 2 encodes proteins needed for other aspects of the LIYV life cycle. LIYV RNA 1 ORF 2 encodes P34, a trans enhancer for RNA 2 accumulation. Here we show that P34 is a sequence non-specific ssRNA-binding protein in vitro. P34 binds ssRNA in a cooperative manner, and the C-terminal region contains the RNA-binding domain. Topology predictions suggest that P34 is a membrane-associated protein and the C-terminal region is exposed outside of the membrane. Furthermore, fusions of P34 to GFP localized to the perinuclear region of transfected protoplasts, and colocalized with an ER-specific dye. This localization was of interest since LIYV RNA 1 replication (with or without P34 protein) induced strong ER rearrangement to the perinuclear region. Together, these data provide insight into LIYV replication and possible functions of P34.

Copyright © 2010 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.virol.2010.04.006
Version: za2963e q8za4 q8zba q8zc7 q8zd5 q8ze3 q8zf7 q8zg6

Similar articles you may find interesting…

  1. Plant-based rapid production of recombinant subunit hemagglutinin vaccines targeting H1N1 and H5N1 influenza.
    Yoko Y Shoji, Jessica A JA Chichester, ... Vidadi V Yusibov

    Hum Vaccin (2011) PMID 21266846

    We describe the large-scale production of recombinant hemagglutinin proteins from A/California/04/09 (H1N1) and A/Indonesia/05/05 (H5N1) strains of influenza virus in Nicotiana benthamiana plants, and their immunogenicity (serum hemagglutination inhibition and virus neutralizing antibodies), and saf...
  2. Antibodies to plant-produced Plasmodium falciparum sexual stage protein Pfs25 exhibit transmission blocking activity.
    Christine E CE Farrance, Jessica A JA Chichester, ... Vidadi V Yusibov

    Hum Vaccin (2011) PMID 21266847

  3. Co-expression of multiple target proteins in plants from a tobacco mosaic virus vector using a combination of homologous and heterologous su...
    Gourgopal G Roy, Sangeetha S Weisburg, ... Vidadi V Yusibov

    Arch Virol 156(11):2057-61 (2011) PMID 21779909

    We engineered a single-component chimeric tobacco mosaic virus (TMV)-based vector containing homologous and heterologous capsid protein subgenomic RNA promoters. Delivery of this vector into Nicotiana benthamiana plants via agroinfiltration resulted in co-expression of two reporter genes within a si...