Advanced search×

Isolation of quality total RNA from the aromatic plant Origanum onites.

Ismail I Poyraz, Emel E Sözen, and Muhittin M Arslanyolub

Z Naturforsch C 65(3-4):266-70 (2010) PMID 20469647

We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and it is fast, simple, and effective. The procedure can be completed within 3 hours and may be applicable to other aromatic medicinal plants containing high amounts of phenolic compounds.

Version: za2963e q8za2 q8zb0 q8zc9 q8zd2 q8ze3 q8zf1 q8zga

Similar articles you may find interesting…

  1. Equilibrium size distribution function of clusters in finite system
    IH Umirzakov

    arXiv:1306.0087 [cond-mat.stat-mech] 1 Jun 2013

    The equilibrium size distribution function of clusters (nanoparticles) in the system of finite number of molecules (atoms) in finite closed volume with constant total energy (isolated system) is found using methods of statistical thermodynamics. The distribution function is found from kinetic equat...
    View PDF
  2. Genetic characterization of a core collection of flax (Linum usitatissimum L.) suitable for association mapping studies and evidence of dive...
    Braulio J Soto-Cerda, Axel Diederichsen, and Sylvie Cloutier

    BMC Plant Biol 13(1):78 (2013) PMID 23647851

    Based on the abundant genetic diversity, weak population structure and relatedness and relatively fast LD decay, we concluded that this core collection is suitable for AM studies targeting multiple agronomic and quality traits aiming at the improvement of flax as a true dual purpose crop. Our genomi...
    PDF not found
  3. HCV Envelope protein 2 sequence comparison of Pakistani isolate and In-silico prediction of conserved epitopes for vaccine development.
    Sobia Idrees, Usman A Ashfaq, and Saba Khaliq

    J Transl Med 11(1):105 (2013) PMID 23631455

    HCV E1 protein modeling was performed with I-TASSER online server and quality of the model was assessed with ramchandran plot and Z-score. A total of 3 B-cell and 3 T-cell epitopes were found to be highly conserved among HCV 3a and 1a genotype. CONCLUSION: The present study revealed potential conser...
    PDF not found