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Development of microsphere-based multiplex branched DNA assay for detection and differentiation of avian influenza virus strains.

Wonhee W Cha, Yunqing Y Ma, Yehia Mo YM Saif, and Chang-Won CW Lee

J Clin Microbiol 48(7):2575-7 (2010) PMID 20484609 PMCID PMC2897486

We developed and evaluated a multiplex branched DNA assay for the detection and subtyping of avian influenza (AI) virus strains. The assay successfully detected all 94 AI virus strains of 15 different hemagglutinin (HA) subtypes tested while simultaneously differentiating 24 North American H5, 11 Eurasian H5, and 11 H7 strains. Our study demonstrates for the first time that a branched DNA method can detect targets that show a great amount of sequence variation.

DOI: 10.1128/JCM.01979-09
Version: za2963e q8za5 q8zb3 q8zc3 q8zdd q8ze7 q8zf1 q8zg3
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