Advanced search×

Photoallergenic potential testing by online irradiation and HPLC.

S S Schröder and P P Surmann

Pharmazie 65(5):339-42 (2010) PMID 20503925

A simple screening method was developed for testing the photoallergenic potential of drugs. The photolysis of the drugs under UV-A irradiation was investigated in aqueous buffer solution alone and in combination with a model dipeptide. As an useful tool for this, high performance liquid chromatography (HPLC) based on an automated column-switching system with aqueous online UV-A irradiation was used. For testing the system, hydrochlorothiazide (HC) with well-known photoallergenic properties was used as a drug and alanyltryptophane (ALATRP) as a model for proteins. Promethazine (PMZ) as a second drug was investigated by the developed screening system. The screening was performed at pH 3 and pH 7. Both cleavage of the peptide binding and formation of new products could indicate a photoallergenic potential.

Version: za2963e q8za6 q8zb2 q8zca q8zd3 q8zeb q8zf8 q8zg7

Similar articles you may find interesting…

  1. Implementing an aim-based outcome measure in a psychoanalytic child psychotherapy service: Insights, experiences and evidence.
    Ricky Emanuel, Jocelyn Catty, and Helen Muller

    Clin Child Psychol Psychiatry (2013) PMID 23661788

    We describe the use of an aim-based outcome measure used in routine outcome monitoring of child and adolescent psychotherapy within a child and adolescent mental health service. We aim to explore the clinical feasibility and implications of the routine use of this measure. We argue that use of the m...
  2. Assessing the quality of life of health-referred children and adolescents with short stature: development and psychometric testing of the Qo...
    Monika Bullinger, Julia Quitmann, and John E Chaplin

    Health Qual Life Outcomes 11(1):76 (2013) PMID 23648112

    The QoLISSY questionnaire is a promising step forward in assessing the impact of dSS on HrQoL. It is based on items generated from the subjective experience of short stature children referred for endocrine investigation, is validated for use in five languages and it is easy to administer in clinical...
    PDF not found
  3. Characterization of Periodontal Structures of Enamelin Null Mice.
    Hsun-Liang Chan, William V Giannobile, and Jan Cc Hu

    J Periodontol (2013) PMID 23646854

    Radiographically, the enamel layer was absent in Enam(-/-) mice. Interproximal open contacts were observed exclusively in Enam(-/-) mice and the prevalence decreased over time, suggesting a shifting of tooth position had occurred. Additionally, in the two Enam(-/-) groups, RBH was significantly lowe...